Biological Activity of Different Batches of Equine Chorionic Gonadotropin as Determined by Reversed-phase High-performance Liquid Chromatography and in vivo Assay
Journal Title: Journal of Advances in Medical and Pharmaceutical Sciences - Year 2017, Vol 12, Issue 2
Abstract
Aims: To evaluate the physicochemical profile of commercial batches of eCG, in order to find if differences can be related to their biological activity. Study Design: Commercial eCG was analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) and in vivo bioassay. Place and Duration of Study: Department of Biotechnology (IPEN-CNEN) and Animal antibody production Laboratory (Animal Science Institute), between June 2013 and April 2014. Methodology: Two commercial eCG batches for veterinary use (I and II) and an eCG official International Standard from the World Health Organization (WHO) were analyzed by RP-HPLC. Additionally, two experiments were designed to validate the biological activity. In experiment 1, groups of prepubertal 21–25 day old Wistar female rats (n = 6/group) received the equivalent to 0 UI (saline) and 10 IU of eCG of each one of these preparations. Autopsy was performed 48 h later and ovaries were removed and weighed. The experiment 2 was designed to determine whether increasing the dose of less active eCG batches could increase the ovarian response. Therefore, groups of prepubertal rats (n = 6/group) were treated with 10 and 30 IU eCG from batch II, while eCG from WHO (10 IU) and saline were control. The evaluation of ovarian response was done similar to Experiment 1. Differences among treatments were analyzed by one-way ANOVA. Results: Results of RP-HPLC showed differences in the main tR peak profile (tR 26.7) of the standard WHO compared with eCG batches I and II. In experiment 1, the average ovarian weight of rats treated with eCG from WHO (60.0 ± 12.1 mg) was higher (P < .01) than saline (23.1 ± 1.6 mg) and batches I (37.6 ± 1.4 mg) and II (31.0 ± 4.3 mg). In experiment 2, the ovarian weight of rats treated with 30 IU of eCG of batch II (45.7 ± 4.1 mg) was higher (P < .01) than saline (32.6 ± 1.4 mg) and significantly lower (P = .05) than 10 UI of the standard WHO (63.3 ± 8.1 mg). Conclusion: The low ovarian response to eCG treatments can be related to differences in the physicochemical profile of eCG batches and RP-HPLC is a fast and reliable tool for detecting these differences.
Authors and Affiliations
Rafael Herrera Alvarez, Fabio L. Nogueira Natal, Beatriz E. Almeida, João E. Oliveira, Alfredo J. Ferreira Melo, Maria T. Carvalho Ribela, Paolo Bartolini
Keywords
Related Articles
- EP ID EP311590
- DOI 10.9734/JAMPS/2017/31956
- Views 117
- Downloads 0
The Outcomes of Urethroplasty for Hypospadias Repair in M. Djamil Hospital, Padang, Indonesia
Background: Hypospadias is believed to result from arrested penile development, leaving a proximal urethral meatus. It has challenged generations of reconstructive surgeons trying to extend the urethra with the goal of r...
Analysis of O6-Methylguanine in Cancer Patient Blood during Administration of Cyclophosphamide Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry
Aims: To determine O6-Methylguanine in Cancer Patient Blood during Administration of Cyclophosphamide using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry. Place and Duration of Study: Dharmais Can...
Hydroalcoholic Extract of Alstonia scholaris Arrests Invasion of Plasmodium falciparum by Effective RBC Membrane Stabilization
Aim: Traditionally medicinal plants play a key role in malaria control. Several studies have demonstrated that alkaloids have excellent antiplasmodial potential. Alstonia scholaris is rich in alkaloid content; despite th...
Bioactivity and Phytochemical Screening of Nigerian Medicinal Plants Growing in Ondo and Ekiti State against Bacterial Isolates from Pediatrics Hospital
The Aqueous, ethanol, hexane and ethyl acetate extracts of the leaves, bark and roots of Acacia albida, Anchomanes difformis, Boscia senegalensis, Bridelia ferruginea, Ficus ingens, Indigofera arrecta and Moringa oleifer...
Safety Evaluation of Giant African Land Snails (Archachatina maginata) Haemolymph on Hematological and Biochemical Parameters of Albino Rats
The safety of Archachatina maginata haemolymph on biochemical and hematological indices of albino rats was investigated. Twelve white albino rats were grouped into 3 (A-C) of 4 animals each. Group A rats serve as control...