BIOLOGICAL EVALUATION OF IN VIVO DIURETIC AND ANTIUROLITHIATIC ACTIVITIES OF LEAF EXTRACTS OF MELOCHIA CORCHORIFOLIA
Journal Title: International Journal of Pharmacognosy - Year 2017, Vol 4, Issue 7
Abstract
The present study was investigated to establish the diuretic and antiurolithiatic activities of chloroform and ethanolic leaf extract of Melochia corchorifolia. The chloroform and ethanolic leaf extract were administered to experimental rats orally at a dose of 200 mg/ kg and 400 mg/kg. Furosemide (5 mg/kg) was used as reference standard for diuretic activity. The parameters measured for diuretic activity was total urine volume; urine electrolyte concentrations such as sodium, potassium, chloride and bicarbonates. In in-vitro antiurolithiatic activity, Calcium oxalate crystallization was induced by the addition of 0.01M sodium oxalate solutions in synthetic urine. The effect of chloroform and ethanolic leaf extract of Melochia corchorifolia (100, 200 and 500 μg/ml) was studied by time course measurement of absorbance at 620 nm for ten minutes by means of a spectrophotometer. Both the extracts show good in-vitro antiurolithiatic activity. In in-vivo antiurolithiatic activity, urolithiasis was induced in male rats by administering ethylene glycol (0.75% v/v) in drinking water for 28 days and the parameters such as oxalate, calcium and phosphate were estimated in urine. Serum creatinine, calcium and uric acid were also estimated. Treatment with the leaf extract of Melochia corchorifolia restored all biochemical, urinary parameters. The results obtained justified the importance of the leaf extract of Melochia corchorifolia as a diuretic and antiurolithiatic agent.
Authors and Affiliations
M. N. Palaksha et al.
Keywords
Related Articles
- EP ID EP428713
- DOI -
- Views 76
- Downloads 0
POLY HERBAL THERAPY IN OINTMENT BASE FOR WOUND HEALING ACTIVITY IN RATS
In the present study, an indigenous herbal formulation containing the ethanolic extracts of rhizomes of Curcuma longa, fruits of Terminalia chebula, leaves of Azadirachta indica, leaves of Lawsonia inermis and leaves of...
ANTIOXIDANT ACTIVITY BY DPPH RADICAL SCAVENGING METHOD OF METHANOLIC EXTRACT OF N- BUTANOL FRACTRION OF TRIBULUS TERRESTRIS LINN. (FAMILY ZYGOPHYLLACEAE)
In this study Anti oxidant activity was performed by DPPH(1,1 diphenyl-2-picryl hydrazyl) radical scavenging method for Tribulus terrestris L. Whole plant methanolic extraction fractionation with toluene and n-butanol i...
ANIOXIDANT ACTIVITIES OF SULFATED POLYSACCHARIDE OBTAINED FROM RED ALGAE CORALLINA OFFICINALIS
Sulfated polysaccharides (SP) were extracted from Corallina officinalis marine red algae seaweed by using hydrothermal processes at 100 °C. The antioxidant activity of C. officinalis sulfated polysaccharide were determin...
ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ROOT LEAVES AND FLOWERS OF CICHORIUM INTYBUS
A study was conducted to assess root, leaves and flowers extracts of cichorium intybus for their antimicrobial activities (against four bacterial and fungal strains), antioxidants activities (DPPH, ABTS H2O2 and reduci...
AN EVALUATION OF THE ANTIOXIDANT PROPERTIES OF SOME OIL YIELDING LAMIACEOUS PLANTS FROM MORNI HILLS (HARYANA, INDIA)
The present study was conducted to explore the antioxidant and radical scavenging activities of essential oil extracted from the leaves of plants of family Lamiaceae from Morni hills in the north-eastern part of the stat...