BIOLOGICAL PROPERTIES OF RISOSPHERIC BACTERIA PSEUDOMONAS PUTIDA AND THEIR BIOLOGICALLY ACTIVE METABOLITES IN REGULATION OF PLANT MICROBIAL SYSTEMS
Journal Title: Наукові доповіді НУБіП України - Year 2019, Vol 1, Issue 1
Abstract
Purpose. Study of the cultural-morphological and physiological and biochemical properties of Pseudomonas putida collection strains and their possible use in biotechnological developments and plant protection technologies. Methods. Biotechnological methods were used (obtaining and subcultivation of strains P. putida 8617, 8618, 8619, 8672 in vitro), microbiological (obtaining an isolated colony of bacteria, studying the growth of microbes on cups, studying the nature of growth on beveled agar, morphological, cultural, biochemical and physiological properties of experimental test cultures), statistical methods, light microscopy.. Results. The conducted experiments showed that all the experimental strains Pseudomonas putida were homogeneous, not contaminated and not mutated, retained their cultural-morphological and physiological and biochemical properties. It was determined that the collection strains Pseudomonas putida, which for a long time remained in the collection of IVB (Institute of Microbiology and Virology named after DK Zabolotny), have retained their basic biological properties and can be used for further microbiological and biotechnological research. Conclusions. According to the results of research and analysis of literary sources, Pseudomonas putida has been shown to differ from other typical representatives of its kind. The obtained results are completely consistent with the data presented in the literature on the absorption of carbon and nitrogen, as the only source of power in the taxonomy of experimental crops
Authors and Affiliations
M. V. Patykа, N. V. Zhitkevich, T. V. Ivanova, T. V. Tarasyuk, V. P. Patyka
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