“BIOPRODUCTION OF INDOLE-3-ACETIC ACID IN PRESENCE OF VITAMIN B6 AND B12 BY BACILLUS SUBTILIS AND BACILLUS AMYLOLIQUEFACIENS”
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 2
Abstract
The present study is aimed to investigate the effect of two water soluble vitamins namely Vitamin B6 and B12 on Indole-3-acetic acid (IAA) production by Bacillus amyloliquefaciens and Bacillus subtilis under in vitro condition, in L-tryptophan independent condition. IAA production was checked in presence of Vitamin B6 and B12 at a concentration of 0, 50 and 100 µg/ml in JNFb- minimal medium additionally containing 2.5mM ammonium chloride. Quantitative estimation of IAA production was carried out after 96h at 370C. Vitamin B6 followed by Vitamin B12 acts as the most preferred source for IAA production. Among the five strains, Bacillus Subtilis strain name WR-W2 was the most efficient strain in terms of its capability to produce IAA in presence of vit.B6. However, the level of IAA was very low in presence of vitamins as compared to only tryptophan added medium. Taken together, the result shows that vit.B6 and vit.B12 significantly stimulate IAA production by Bacillus sp. Altogether; the strains might be implicated toward sustainable agriculture.
Authors and Affiliations
VIJENDRA KUMAR MISHRA, ASHOK KUMAR
Keywords
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