Biosafety Assessment of Petroleum Ether Oil of Aframomum melegueta K. Schum in Wistar Rats
Journal Title: Journal of Applied Life Sciences International - Year 2016, Vol 5, Issue 4
Abstract
Objectives: The study was aimed at evaluating the biosafety of reported insecticidal potency of Aframomum melegueta petroleum extract (AMPE) used on stored products using wistar rats as a model. Study Design: Assessment of biochemical indices on treated and control rats. Place and Duration of Study: Department of Biology Federal University of Technology, Akure, Nigeria. April, 2015. Methodology: Aframomum melegueta seeds were extracted using soxhlet extractor and the filtrate later concentrated using the rotary evaporator to obtain A. melegueta petroleum ether extract (AMPE). Twenty four rats were divided into four groups labeled I, II, III and IV (six rats per group). Group I served as the control group and were administered orally with Dimethyl Sulphoxide (DMSO) and normal saline, Group II were administered orally with 500 mg/kg of AMPE, Group III were administered orally with 1000 mg/kg of AMPE while Group IV were administered orally with 2000 mg/kg of AMPE all dissolved in DMSO and Normal saline. Animals were weighed, anesthetized and blood samples were collected at the end 5 days of oral administration. Blood samples were collected by cardiac puncture for some biochemical parameters such as Total proteins, bilirubin, cholesterol, uric acid, creatinine, Urea and Uric acid activities as well as serum, AST and ALT were determined. Results: At the end of this research work, it was discovered that at doses higher than 500 mg/kg there were enzymatic leakages from the organs (kidney and liver) into the serum, compared to the control group. Conclusion: The study revealed that it is advisable to use minimal dosages for the management of insect pest on products meant to be consumed by human, to prevent damage to vital organs as observed in the results at higher doses while higher doses should be for seeds meant for propagation.
Authors and Affiliations
Adenike Christy Adeyemo, Sunday Idowu Oyeleye, Adelowo Adefisayo Adewoyin
Keywords
Related Articles
- EP ID EP351938
- DOI 10.9734/JALSI/2016/26105
- Views 95
- Downloads 0
Water Quality Assessment of the New Calabar River
Water is one essential natural resource whose quality must be periodically assessed and managed. This study was conducted within the Choba portion of the New Calabar River. Surface water samples were collected from three...
Phenotypic Correlations between External and Internal Egg Quality Traits of Coturnix Quails Reared under Intensive Housing System
Aims: The present study investigated phenotypic correlations between external and internal egg quality traits in Japanese quail. Study Design: Laboratory analysis was carried out using sensitive scale and Vernier calipe...
Evaluation of Biochemical and Physiological Parameters of the Leaves of Tree Species Exposed to Vehicular Emissions
Aims: To determine the sensitivity and tolerance levels of the tree species to air pollution using biochemical and physiological parameters. Study Design: Field and Laboratory Studies Place and Duration of the Study: Thr...
Post-traumatic Stress Disorder amongst Internally Displaced Pregnant Females
Aims: The study was aimed to assess the association of post-traumatic stress disorder with pregnancy and birth outcomes amongst pregnant females internally displaced from the south-east of Ukraine. Study Design: Observa...
Study on the Prevalence of Fascioliasis on Cattle Slaughtered at Minna Modern Abattoir, Niger State, Nigeria
Aims: Fascioliasis is a helminthic disease caused by trematodes of the genus Fasciola. This study determined the prevalence of fascioliasis on cattle slaughtered at Minna Modern Abattoir, Tayi village, Bosso Minna, Niger...