Biosynthesis of Copper nanoparticles using bacterial supernatant optimized with certain agro-industrial byproducts
Journal Title: Novel Research in Microbiology Journal - Year 2019, Vol 3, Issue 6
Abstract
Biosynthesis of green nanomaterials using microorganisms is considered clean, eco-friendly and viable, instead of the physical or chemical methods. This study aimed in the biosynthesis of copper nanoparticles (CuNPs) exploiting Egyptian local bacterial isolates. Sixteen copper-resistant isolates out of 160 bacterial isolates; were captured from various plant rhizospheres including; chamomile, hibiscus, neem, iris, and pea, beside samples collected from the Sharm El-Sheikh seawater. Among the 16 copper-resistant isolates, one promising isolate I108 was chosen which synthesized CuNPs of diameter about 87.1 nm, showed UV absorbance of 0.54 at 580 nm, with a concentration of 12.21 mg\ l. This isolate was characterized by phenotypic and genotypic features. Based on 16S rRNA gene analysis and compared with the sequences presented in NCBI GenBank, the phylogeny positions assessment confirmed that it belonged to Genus Pseudomonas, and was closely related to Pseudomonas silesiensis strain A3 (98% similarity). For the bacterial synthesis of CuNPs, optimization of the P. silesinsis strain A3 cell-free supernatant was carried out using seven agro-industrial residues, added to the basal medium as different carbon sources. Results showed that 2% blackstrap sugar cane molasses was the most efficient carbon source for CuNPs biosynthesis, when incubated for at 30°C for 24 h using shaking speed of 120 rpm. The biosynthesized CuNPs has a size of 66.12 nm at a concentration of 19.2 mg\ l, and maximum surface plasmon peak (SPR) of 0.85.
Authors and Affiliations
Abd-Elhalim, B. T. ; Gamal, R. F. ; Abou-Taleb, Kh. A. ; Haroun, A. A.
Keywords
Related Articles
- EP ID EP691313
- DOI 10.21608/nrmj.2019.66748
- Views 103
- Downloads 0
Genetic diversity of Fusarium solani f.sp. cucurbitae the causal agent of crown and root rot of watermelon in Tunisia using ISSR markers
Fusarium solani f.sp. cucurbitae (F.s.c.) Snyder and Hansen is one of the most important fungi causing serious damages to the cucurbits production areas in Tunisia. The goal of this investigation was to study the genetic...
Induction of defence enzymes activities in grape plant treated by seaweed algae against Plasmopara viticola and Uncinula necator causing downy and powdery mildews of grapes
Plasmopara viticola and Uncinula necator are the causative agents of downy mildew and powdery mildew diseases, respectively, which become a major problem in grape crop (Vitis vinifera L.). The use of natur...
Efficacy of Pseudomonads as biocontrol agents of phytopathogens
Microbial plant pathogens infecting different crops cause great economic losses worldwide. Thus plant diseases need to be controlled to maintain the quality and abundance of these crops...
Pathogenicity and inoculum concentration effects of Clavibacter michiganensis subsp. michiganensis on severity of bacterial canker of tomato
A total of 100 tomato plant samples exhibiting typical symptoms of bacterial canker were collected from tomato-growing areas of north-western areas of Pakistan. On isolation of the causal agent of this di...
Detection of Cucumber mosaic cucumovirus in infected cowpea plants (Vigna unguiculata L.) from northern Egypt
Cucumber mosaic cucumovirus (CMV) was isolated from naturally infected Cowpea plants (Vigna unguiculata L.) showing different symptoms of mosaic; mottle, dwarfing, and vein clearing, grown at certain locations of Alexand...