Biotechnological Production of α-Amylase through Submerged Fermentation by Bacillus Megaterium Klmi4 using Agro-Wastes as Substrate
Journal Title: International Journal of Environment, Ecology, Family and Urban Studies (IJEEFUS) - Year 2019, Vol 9, Issue 3
Abstract
Different agro-wastes (rice husk, groundnut oil cake and green gram husk) with different quantities (1, 3, 5, 7 and 10 g) were inoculated with 1 ml of bacterial suspension and incubated at 37 0C (room temperature) in rotary shakers. At every 24 h intervals samples were withdrawn from each flask and amylase production was estimated. The highest yield of 9 IU was recorded with 5 g concentration in case of rice husk at 48. Similarly 10 IU and 42 IU was recorded with 5 g concentration at 48 h in both the cases of Groundnut oil cake and Green gram husk respectively. Total carbohydrate, total protein, and total fats were estimated of three agro-wastes. Basal parameters (pH, Temperature, and Inoculum size) were optimized. Enzyme activity increased up 75 IU at pH 8.0, 125 IU at 370 and at 3.0x106cfu/ml, the highest enzyme activity of 128 IU was observed after 48 h incubation.
Authors and Affiliations
Masarath Irfana
Keywords
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- EP ID EP608442
- DOI 10.24247/ijeefusjun20195
- Views 93
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