Biovalorization of Olive Mill Waste Water for the Production of Single Cell Protein from Saccharomyces cerevisiae, Candida utilis and Pleurotus ostreatus
Journal Title: Biotechnology Journal International - Year 2016, Vol 11, Issue 2
Abstract
Aims: The aim of this work was to investigate and optimize the potential of olive mill waste water (OMWW) to be utilized as a substrate for the production of single cell protein (SCP). Study Design: The study was divided in two phases, a preparatory phase for the removal of olive polyphenols and/or condensation of OMWW, and a bioprocessing-fermentation phase. Place and Duration of Study: The study was conducted at the TEI of Thessaly from January 2013 to August 2015. Methodology: OMWW was used as a fermentation substrate for production of SCP after dephenolization by microfiltration, condensation via reverse osmosis, and addition of minerals/nitrogen sources. Saccharomyces cerevisiae (S. cerevisiae), Candida utilis (C. utilis) and Pleurotus ostreatus (P. ostreatus) were grown in this substrate under controlled process conditions in shake flasks and a 15 lt bioreactor. Biomass, sugars, phenol concentration of OMWW and the protein content of the harvested biomass were evaluated. Results: Dephenolization of OMWW is essential for sufficient growth of both yeasts, while P. ostreatus grows better in the untreated substrate, as it can degrade the polyphenols while the dephenolization step reduced also the sugar content of OMWW. Optimum process conditions for S. cerevisiae included a pH 7, 350 rpm agitation rate, addition of (NH4)2SO4 to a 3/1 condensed medium, while C. utilis grew best at pH 5, 250 rpm, after addition of NH4NO3 in a 3/1 condensed medium. Maximum biomass concentration was 13.5 g/l and 14.6 g/l, respectively. 14.8 g/l of P. ostreatus biomass were obtained at pH 5, 150 rpm after addition of NH4NO3. This mold had the highest yield but lowest productivity due to slow growth. Conclusion: P. ostreatus is a good producer of SCP in OMWW and reduces its organic load, but it has lower protein content. C. utilis had the highest SCP productivity, and the two yeasts had the highest protein concentration but require dephenolization of OMWW.
Authors and Affiliations
Ioannis Giavasis, Konstantinos Petrotos
Keywords
Related Articles
- EP ID EP237729
- DOI 10.9734/BBJ/2016/22509
- Views 97
- Downloads 0
Antibacterial and Hemagglutinating Activity of the Fruit Pulp of Trilepisium madagascariense DC
Aim: This study aimed at exploring the hemagglutinating and antibacterial activities of crude lectin from the fruit pulp of Trilepisium madagascariense DC. Methods: Crude lectin was obtained by maceration of the fruit a...
Assessment of Antiproliferative Potential of Hexalobus crispiflorus (Annonaceae)
Background: Hexalobus crispiflorus root aqueous extract (HC) is used to treat breast cancer in Nigeria. Aim: To evaluate the antiproliferative effects of HC using Sorghum bicolor seed radicle as test subject. Materials...
Histopathological Effects of Glyphosate and Its Toxicity to the Earthworm Nsukkadrilus mbae
This study was carried out to investigate the toxicity of Roundup, aglyphosate-containing herbicide to the earthworm Nsukkadrilus mbae and the associated histological changes. One hundred and sixty five earthworms were r...
Direct Multiple Shoot Induction and Plantlet Regeneration from Cotyledonary Explants of Sapindus emarginatus Vahl (Soapnut)
Multiple shoot induction in S. emarginatus has been achieved by two methods: (1) Direct germination of S. emarginatus in vitro cotyledon explants in BAP/Kn/TDZ (1.0-3.0 mg/L) supplemented MS medium and (2) in plant treat...
Phosphate-Solubilizing Bacteria in the Rhizosphere of Some Cultivated Legumes from Meknes Region, Morocco
Aims: Through the National Botanical Research Institute's phosphate growth medium (NBRIP) and 16S rDNA sequence analysis were used to isolate and identify the bacterial groups that actively solubilized phosphates in vitr...