Bisphenol-A and Hexachlorocyclohexane Induced Changes in Gonadal Sex Hormones in an Indian Major Carp, Labeo rohita (Ham.1822)
Journal Title: International Journal of Zoological Investigations - Year 2017, Vol 3, Issue 1
Abstract
This study presents the effects of different water concentrations of Bisphenol A (BPA) and Hexachlorocyclohexane (γ-HCH) on a fresh water fish Labeo rohita, commonly found in rivers of India. Total of 200 immature sexually undifferentiated fishes were collected, acclimatized to laboratory conditions and kept in glass aquaria containing 0.2, 0.3, 0.5 mg/L of either BPA or γ-HCH for 30, 60, 90 days along with corresponding controls. At each interval the length, weight, gonado-somatic index (GSI) and RNA/DNA ratio from fish muscle were determined. Serum levels of estradiol, testosterone, aromatase and vitellogenin was measured by sandwich ELISA. Gonads were processed for histological studies. Results at statistical significance level of p<0.05 delineated depressed length, weight, GSI and RNA/DNA ratio. BPA treated fish had significantly (P<0.001) reduced serum testosterone (531.25± 19.9 pg/ml) level. However, in case of γ-HCH treatment serum testosterone increased (1933.2±14.5pg/ml). In BPA treated fish serum 17-β estradiol significantly (P<0.001) increased (1088±18.5 pg/ml) but γ-HCH treatment significantly (P>0.05) decreased the value as compared to control. Further, serum vitellogenin increased ten times (10323± 20.0 pg/ml) after high dose of BPA treatment while γ-HCH treatment slightly depressed it. There was increased (P<0.001) serum aromatase levels (213.5± 8.9 pg/ml) after BPA and γ-HCH treatments. The testes of BPA exposed fish showed disorganized tubular elements, degenerated seminiferous tubules and interestingly developing oocyte and a clear mature oocyte (OC). The results of this study suggest that BPA and γ-HCH caused hormonal imbalance leading to endocrine disruption.
Authors and Affiliations
A. K. Singh
Keywords
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