Blood group serology and gene detection in patients with B(A) subgroup in Jiaozhou, Qingdao
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 3
Abstract
Objective To investigate and analyze the serological and molecular biological characteristics of B(A) subgroup in a tertiary hospital in Jiaozhou, Qingdao. Methods From November 2019 to February 2023, the samples of 12 patients were suspected to be AB subgroup by microcolumn glass bead method and saline test tube method. The exons 6 and 7 of ABO gene were further amplified, sequenced and analyzed to determine the ABO allele type. Results A total of 9 cases of B(A) subgroup were detected in 26 065 patients in Jiaozhou, with a detection rate of 0.345 ‰ ( 9/26 065 ). Among the 9 cases of B(A) subgroup, 8 cases of serological reaction showed AweakB, and the gene detection was heterozygous for BA.04 gene and O gene.One case of serological reaction showed ABweak, and the gene detection was heterozygous for BA.04 gene and A gene. Conclusion Blood group serological combined with gene detection can accurately identify ABO blood group. B(A) subgroup alleles can exist in individuals with serological reaction of ABweak.
Authors and Affiliations
Hongwei QIN, Xiaoxia WANG, Ruilan YIN, Shuchao ZHANG
Keywords
Related Articles
- EP ID EP738784
- DOI 10.13303/j.cjbt.issn.1004549x.2024.03.011
- Views 36
- Downloads 0
The introduction to and inspiration from haematological management of major haemorrhage: a British Society for Haematology guideline
Evidenced based and practical guidelines have important role in the decrease of mobility and mortality of the patients with major haemorrhage. Recently, British Society for Haematology updated a guideline on haematologic...
Retrospective analysis and prevention strategies of blood-borne occupational exposure in Beijing Red Cross Blood Center from 2010 to 2021
Objective To understand the occupational exposure of blood center staff during blood collection and supply, analyze the influencing factors, explore preventive measures and improve the self-protection awareness of staff,...
Desalination effect on FⅧ components: a compartive study among 5 desalination methods
Objective To compare the desalination effects of five desalination methods and their effects on the components for human coagulation factor Ⅷ(FⅧ), and provide reference for selection of protein desalination methods. Meth...
Blood screening results of voluntary blood donors in Xi′an district from 2012 to 2021: a retrospective study
Objective To explore the population characteristics, the reasons for deferral and the changing trend of unqualified rate of blood test of voluntary blood donors in Xi′an, so as to provide a data support for recruiting lo...
The treatment of refractory wounds with apheresis platelet-rich plasma
Objective To explore the model of treatment of refractory wounds by apheresis platelet-rich plasma (PRP), and evaluate its efficacy and safety. Methods PRP treatment was carried out for 34 patients with refractory wounds...