Abstract

The purpose of the study was to evaluate the status of vitamin D in healthy subjects. Vitamin D level was determined on the basis of blood serum 25(OH)D concentrations, taking into account the time of blood collection and age. Moreover, it was to find correlations between serum concentrations of: 25(OH)D, calcium, inorganic phosphates and serum alkaline phosphatase (ALP) activity. The randomized study was carried out on a group of 40 healthy subjects. Each person had venous blood samples drawn twice: from 6 March, 2012 to 15 March, 2012 and from 25 June, 2012 to 6 July, 2012. The results found the majority of subjects had lowered serum 25(OH)D concentrations compared to the reference range determined in the winter months of little insolation and in the summer months of much insolation. In the summer the blood serum concentration of 25(OH)D and ALP activity were statistically significantly higher in comparison with the winter and spring. The concentration of 25(OH)D was not statistically significantly related to age, concentrations of Ca, inorganic phosphates and ALP activity.

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