Body cavity fluid flowcytometry in the diagnosis of haematolymphoid neoplasms
Journal Title: Journal of Medical Science And clinical Research - Year 2018, Vol 6, Issue 9
Abstract
Introduction: Flow cytometry provides a rapid and accurate analysis of haematopoetic tumour cells in serous fluids. Material & Methods: This is a study of body cavity fluids in which flow cytometry was used to characterize the haematopoetic cells. Period of study is from January 2012 to May 2018. Results: 39 patients were included in the study, out of which, there were 8ascitic fluid, 2 CSF (cerebrospinal fluid) and 29 pleural fluid samples. Among the asciticfluid samples, most common cases were of Burkitt lymphoma. In the 2 CSF cases, one was a rare case of leptomeningeal Diffuse Large B cell lymphoma and the other was a reactive lymphoid population. Among the pleural fluids, most common was T- Lymphoblastic Lymphoma. Among the pleural fluid samples there were 4 reactive lymphocytosis (3 associated with Classical Hodgkin Lymphoma & 1 with a Primary Mediastinal Large B cell Lymphoma). The 3 benign effusions associated with Classical Hodgkin Lymphoma showed an abnormal increased CD4:CD8 ratio. In addition to flow cytometry, in cases of DLBCL/ Burkitt lymphoma IHC markers like bcl2, bcl6, SIgM, TdT and MIB were done on cell block preparation to corroborate the diagnosis. Conclusion: Flow cytometry helps rapidly and accurately characterize benign and malignant haematolymphoid effusions and in conjugation with immunohistochemistry on cell block preparation obviates the need for a tissue biopsy .The increased CD4 T cell subset in pleural fluid samples of Classical Hodgkin Lymphoma probably corresponds to the increase in T regulatory cells with a CD4, CD25, CD152, and FoxP3 immunophenotype which has been identified in the background T cell population of Classical Hodgkin Lymphoma.
Authors and Affiliations
Dr Rekha. A . Nair
Keywords
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