Calcium antagonist of n-butanol fraction (BuF) from the stem bark of Terminalia superba Engl. et Diels (Combretaceae) on rabbit duodenum
Journal Title: Scholars Academic Journal of Pharmacy - Year 2014, Vol 3, Issue 1
Abstract
The Objective of the present study was to investigate the pharmacological effects and the possible mechanism (s) that mediated the effects of the organic fraction from n-butanol-water partiton (50:50 v/v) of the stem bark of Terminalia superba and to screen for the presence of phytochemical constituents which can be responsible for these observations. Organic n-butanol fraction (BuF) from the stem bark of Terminalia superba was evaluated for its capacity to induce spasmolytic effect on spontaneous contraction of isolated rabbit duodenum as well as for its possible calcium blocking actions. Pieces of rabbit duodenum (3cm) were mounted in an organ bath containing air bubbled Mac Ewen solution with 1g initial tension. The non cumulative concentrations of BuF exhibited a significant (p<0.05) relaxation on the spontaneous contraction of isolated rabbit duodenum for concentrations ranging from 0.02 to 0.08 mg/ml. The pre-contracted duodenum by ACh (2.20x10-6mg/ml), KCl (2.43 x10-6mg/ml) and BaCl2 (2.30 x10-6mg/ml) were relaxed by the non cumulative concentrations (0.02, 0.04 and 0.08 mg/ml) of BuF (p<0.05). In high potassium (2.43 x10-6mg/ml) Ca2+-free Mac Ewen solution, non cumulative concentration of CaCl2 (2.02 x10-3µg/ml) induced duodenum contractions. However, the non cumulative concentrations of BuF (0.02, 0.04 and 0.08 mg/ml) reduced these CaCl2-induced contractions concentration-dependently (p<0.05). These results suggest that BuF antispasmodic effect on rabbit duodenum may be due to blockade of voltage dependent calcium channels. Tannins, flavonoids, quinones, saponins, reduced sugar, sterols and polyterpenes were found out as major active constituents of the tested extract. Some of these phytochemical constituents such as tannins, flavonoids, saponins, sterols and polyterpenes which are known for their antispasmodic effect may explain the relaxant actions of BuF. Keywords: Antispasmodic, Terminalia superba, rabbit, duodenum.
Authors and Affiliations
Nomane Bernard Goze, Kouakou Léandre Kouakou, Nahounou Mathieu Bléyéré, Brou André Konan, Kouao Augustin Amonkan, Kouakou Jean Claude Abo, Ehouan Etienne Ehilé
Keywords
Related Articles
- EP ID EP377341
- DOI -
- Views 122
- Downloads 0
Validation: A Significant Tool for Enhancing Qualities of Pharmaceutical Products
Validation means confirmation of quality by with the documentation system. Quality is the main aspect of every pharmaceutical company and it is achieved with the help of validation process. Validation is the documented e...
Experimental Evaluation of Anticonvulsant Property of Citrus sinenses (Leaf Extract) in Mice
Abstract:Leaf extracts of Citrus sinensis have been used in local folk medicine to treat neurological diseases. Its methanolic extracts have sedative activity. Diosmin and Hesperidine have been shown to posses’ anti-infl...
Phytochemical Screening and Antibacterial Activity of Aqueous Leaf Extract of Santalumalbum Linn
Santalum album Linn is one of the important medicinal plants belonging to the family Santalaceae. Ayurveda and Unani medicinal systems use it for the treatment of several ailments. Santalum album Linn has Anti pyretic, A...
Reviewon Sleep and Sleep Disorders
Sleep is a complex neurological state, with its primary function of providing rest and restoring the body’s energy levels. Normal human sleep is divided into non–rapid eye movement (NREM) and rapid eye movement (REM) sle...
Extraction, Isolation and Antimicrobial Activity of Crude and Purified Ferritin extract from seeds of Soyabean (Glycine max (L.) Merr. )
The present study aimed to isolate, purify and evaluate the antimicrobial activity of ferritin extracted from Soyabean (Glycine max (L.) Merr. ) against Gram-negative microorganisms (Escherichia coli, Pseudomonas aerugin...