Calcium, Magnesium and Total Antioxidant Capacity (TAC) in Seminal Plasma of Water Buffalo (Bubalus Bubalis) Bulls and their Relationships with Semen Characteristics
Journal Title: Veterinary Research Forum - Year 2010, Vol 1, Issue 1
Abstract
In order to determine calcium (Ca), magnesium (Mg) content and total antioxidant capacity (TAC) of seminal plasma in buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen quality was evaluated, seminal plasma was then harvested by centrifugation and its Ca and Mg content were estimated and its TAC determined. The Ca and Mg content of the seminal plasma (Mean ± SEM) were recorded as 22.36 ± 0.52 mg dl[sup]-1[/sup] and 11.94 ± 0.36 mg dl[sup]-1[/sup] respectively, while, its mean TAC value was 1.50 ± 0.02 mmol L[sup]-1[/sup]. The mean Ca value was highly associated with sperm progressive motility, gross motility, viability ([i]P[/i] = 0.000 for all), negatively with semen volume ([i]P[/i] = 0.01), and with Mg and TAC values ([i]P[/i] = 0.000 for both). The mean Mg values was highly associated with sperm progressive motility, gross motility and viability and seminal plasma Ca and TAC ([i]P [/i]= 0.000 for all) and negatively associated with semen volume ([i]P[/i] = 0.014). The mean TAC values was highly associated with sperm progressive motility, gross motility and viability and seminal plasma Ca and Mg ([i]P[/i] = 0.000 for all). For further clarification of these associations, the data was categorized in three groups of excellent (Ex, >90% motile, n = 33), good (Go, 80-89% motile, n = 15) and moderate (Mo, <79% motile, n = 6) according to their percentage of sperm motility. The mean progressive motility in Ex group was 92.24 ± 0.51%, in Go group it was 81.66 ± 0.62 %, and in Mo group it was 71.66 ± 1.05 %. The mean Ca, Mg and TAC values were respectively recorded as 25.12 ± 0.29 mg dl[sup]-1[/sup], 13.78 ± 0.20 mg dl-1, and 1.57 ± 0.009 mmol L[sup]-1[/sup] in Ex, 18.74 ± 0.63 mg dl[sup]-1[/sup], 9.14 ± 0.33mg dl[sup]-1[/sup], and 1.42 ± 0.044 mmol L[sup]-1[/sup] in Go, and 17.34 ± 0.18 mg dl[sup]-1[/sup], 8.06 ± 0.25 mg dl[sup]-1[/sup], and 1.23± 0.05 mmol L[sup]-1[/sup] in Mo groups. The associations in groups are discussed. These results show that seminal plasma Ca and Mg content and TAC are associated with semen characteristics, and synergistically have an effect on motility and viability of the spermatozoa after ejaculation, which are important factors in semen fertility.
Authors and Affiliations
Mahdi Eghbali, Sayed Morteza Alavi-Shoushtari, Siamak Asri-Rezaei, Mohammad-Hassan Khadem Ansari
Keywords
Related Articles
- EP ID EP98072
- DOI -
- Views 149
- Downloads 0
Closing gaps: Integrating food safety management systems into the veterinary curriculum a tool to improve food quality and trade
Detection of tetracycline resistance genes in bacteria isolated from fish farms using polymerase chain reaction
Five common tetracycline resistance genes tet(A), tet(B), tet(M), tet(O) and tet(S) were studied by polymerase chain reaction in 100 bacteria isolated from Iranian fish farms. In the antibiogram test most of the bacteria...
Luteal activity of Abadeh ecotype does in summer and winter and the effect of kisspeptin-10 on luteinizing hormone secretion in the anestrus does
The aims of the present study were to evaluate luteal activity in Abadeh ecotype goat during summer and winter and also the effect of a single dose kisspeptin-10 injection on the secretion of luteinizing hormone (L...
Effect of a phytogenic feed additive on performance, ovarian morphology, serum lipid parameters and egg sensory quality in laying hen
This present study was conducted to evaluate the effects of dietary inclusion of 4, 8 and 12 g kg-1 phytogenic feed additives mixture on performance, egg quality, ovary parameters, serum biochemical parameters and yolk t...
Ectoparasitic infestations of the European hedgehog (Erinaceus europaeus) in Urmia city, Iran: First report
Hedgehogs are small, nocturnal mammals that become popular in the world and have significant role in transmission of zoonotic agents. Some of the agents are transmitted by ticks and fleas such as rickettsial agents. For...