Can p63 Alone Resolve the Mystery of Suspicious Lesions in Prostate Biopsies?-A Study of Interobserver Agreement
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 12
Abstract
ABSTRACT Introduction: Prostatic biopsies more often, present diagnostic dilemma raising the suspicion of malignancy. Small acinar pattern, limited atypical glands and benign mimickers are few pitfalls. Markers like p63, High Molecular Weight Cytokeratin (HMWCK) detect presence/absence of basal cells and p504s, malignant epithelial cells. This study highlights the usefulness of p63 alone in the resolution of suspicious foci. Aim: To ascertain the effectiveness of p63 in resolving doubtful prostatic biopsies. Materials and Methods: Cross-sectional study on 30 prostatic biopsies with suspicious foci from January 2014 to July 2016. Immunohistochemistry with p63, HMWCK and p504s was performed on all the biopsies and final diagnosis was arrived at. The routine paraffin sections and p63 slides were reviewed by two independent pathologists who were blinded to the above diagnosis. Their opinion was analysed against the final diagnosis with SPSS v.23 to calculate sensitivity, specificity and interobserver agreement. Results: The diagnosis achieved by performing three markers were benign in 24 and adenocarcinoma in six cases. Pathologist 1 concurred in 30 (100%) cases and pathologist 2 in 29 (97%). A p63 had (6/6) 100% sensitivity and specificity in diagnosing adenocarcinoma with pathologist 1. With pathologist 2, it had sensitivity of (6/6) 100% and specificity of (23/24) 95.83%. Cohen’s kappa was 0.902 which showed significant agreement. Conclusion: A p63 alone can help the pathologist to arrive at a diagnosis and is highly sensitive and specific. Interobserver agreement is also significant indicating high reproducibility.
Authors and Affiliations
Hema Kini, Beshwanth V Chowdary, Radha R Pai, Jyoti R Kini
Keywords
Related Articles
- EP ID EP525861
- DOI 10.7860/JCDR/2018/37647.12356
- Views 34
- Downloads 0
Pure Primary Extragonadal Retroperitoneal Yolk Sac Tumour in a Young Child: A Case Report
Germ Cell Tumours (GCTs) in children are uncommon, constituting approximately only 3% of all malignancies in children younger than 15 years of age. Primary extragonadal GCTs constitute only 1-5% of all GCTs and a retrope...
Neurocognitive Developmental Outcomes in Early Adolescent Rats Prenatally Exposed to Choline and Docosahexaenoic Acid
ABSTRACT Introduction: Prenatal nutritional status plays a remarkable role in neurocognitive development of an offspring. Although, essential nutrients like iron, calcium, and vitamins are being supplemented during pregn...
Correspondence: Modulation of Lung Function by Increased Nitric Oxide Production
Letter to Editor
Prevalence of Vitamin D Deficiency and its Associated Risk Factors during Early Pregnancy in a Tropical Country: A Pilot Study
ABSTRACT Introduction: Vitamin D deficiency (circulating 25-hydroxyvitamin D level <50 nmol/L) is a major health problem especially among pregnant women because it can affect the health of both mother and foetus. This pr...
Comment on “Serological Evidence of Scrub Typhus among Cases of PUO in the Kashmir Valley- A Hospital Based Study”
Letter to Editor