Candidacidal activity of saliva preparation containing gold nanoparticles
Journal Title: Engineering of Biomaterials / Inżynieria Biomateriałów - Year 2016, Vol 19, Issue 137
Abstract
Natural saliva is one of the most important biological fluids and it fulfils many important functions in oral cavity. However, in some cases, disorders of salivary gland functions may cause hyposalivation (reduction of saliva secretion) or xerostomia (dryness of the oral cavity). Thus, so-called saliva substitute preparations can be used to support the functions of saliva. In our work, saliva substitute was prepared on the basis of porcine gastric mucin and xanthan gum, both dissolved in saline buffer. Additionally, gold nanoparticles were added to tested solution with concentration of 200 μg/ml and biological characterisation was evaluated. Analysis of the candidacidal activity (against Candida albicans) of prepared saliva substitute with addition of gold nanoparticles was performed. Tests were carried out after keeping samples of dental material (Co-Cr-Mo alloy, Remanium 380) in control sample and saliva preparations (with and without gold nanoparticles) during 72 hours at 37°C. After incubation, tested surfaces were observed using confocal laser scanning microscopy (CLSM). The obtained results of microscopic observations showed differences between the tested preparations in the scope of presence of Candida albicans. It was observed that gold nanoparticles significantly reduced the growth of Candida albicans species at tested dental material.
Authors and Affiliations
J. Mystkowska, K. Niemirowicz, G. Tokajuk, D. Łysik, E. Lewkowicz, J. R. Dąbrowski, R. Bucki
Keywords
Related Articles
- EP ID EP559780
- DOI -
- Views 25
- Downloads 0
Evaluation of Candida albicans adhesion on AlSl-316L and Ti6Al4V in changing environmental conditions
Currently, studies on the formation of microbiological biofilm on biomaterial surfaces and medical devices have become an important aspect in medicine. In vivo studies of the biofilm development show a high correlation b...
Wpływ dodatku kwasów karboksylowych na czas wiązania i wytrzymałość na ściskanie cementów szkło-jonomerowych
Cementy szkło-jonomerowe powstają w czasie utwardzania kompozycji złożonych z dwóch składników: szklanego proszku i płynu wiążącego będącego wodnym roztworem polikwasów alkenowych. Wiązanie cementu polega na reakcji kwas...
Genotoxicity of antibacterial bioglasses obtained by sol-gel method for Salmonella typhimurium
Periodontal disease causes problems in dentistry. Surgical intervention with appropriate biomaterials for tissue regeneration is necessary in advanced stages of the disease. Therefore, the aim of this study was to determ...
Modification of PLGA microspheres’ microstructure for application as cell carriers in modular tissue engineering
Microspheres (MS) made of resorbable polymer have been proposed as a cell growth support. They may be assembled to form cell constructs or be suspended in hydrogels allowing injection into injury location. High relative...
The influence of the DLC and DLC-Si coatings on the changes occurring on the implants surface during the implant-bone contact
In this study the DLC and Si-incorporated DLC layers were deposited on stainless steel alloy (AISI 316 LVM) using modified radio frequency plasma assisted chemical vapour deposition (RF PACVD) method and examined in term...