Cardamom protects kidneys and decreases the expression of TRPM2 channels in doxorubicin- induced nephropathy
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2016, Vol 4, Issue 9
Abstract
Doxorubicin (DXR) is a strong antineoplastic that has toxic side effects. In kidneys, DXR has been shown to have injurious effects. We investigated the protective effect of cardamom (CAR) on DXR- induced nephropathy (DIN). Four groups of six adult male Wistar Albino rats each were formed. During the 14- day experimental period, group I received no application, group II received 15 mg/ kg intraperitoneal (IP) dose of DXR, group III received 15 mg/ kg IP dose of DXR and 500 µl/ kg of CAR orally per day, and group IV received 500 µl/ kg of CAR orally per day. Transient receptor potential melastatin 2 (TRPM2) immunoreactivity, apoptosis by TUNEL staining, and malondialdehyde (MDA) levels were determined. The MDA levels of the DXR group were significantly higher than the control and CAR groups. The MDA levels of the DXR+ CAR group have significantly decreased in comparison with the DXR group. The prevalence of TRPM2 immunoreactivity was +1 in the control and CAR groups, +3 in the DXR group, and decreased to +1 in the DXR+ CAR group. Apoptosis in kidney tissue determined by TUNEL staining was similar in the control and CAR groups, but compared to the control group, there was a significant increase in the DXR group and and a significant decrease was observed in the DXR+ CAR group. We determined that CAR reduced MDA levels, apoptosis, TRPM2 expression and had potential antioxidant effects in DIN, and may be considered a preventative agent. Keywords: Doxorubicin, apoptosis, nephropathy, TRPM2, cardamom
Authors and Affiliations
Ali Gurel, Hasan Atli, Tuncay Kuloglu, Ebru Onalan, Nalan Kaya, Huseyin Celiker
Keywords
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