Cathepsin mRNA level in bovine cumulus cells fails to be a good marker of oocyte quality
Journal Title: Animal Science Papers and Reports - Year 2016, Vol 34, Issue 4
Abstract
Cathepsins (CTS) are lysosomal cysteine proteinases, of which elevated transcript content is attributed to the reduced quality of oocytes. The aim of this study was to investigate whether transcript content of three CTS genes in cumulus cells (CC) may be related to oocyte quality. The relationships between mRNA content of CTS genes in CC, the fatty acids profile in follicular fluid (FF) and selected quality traits of the corresponding oocyte (COC morphology, follicle diameter, mtDNA copy number) were investigated. Although an increased RA of the CTSB and the CTSK genes was accompanied by inferior COC morphology, it was also correlated with a higher mtDNA copy number in the oocyte and an increased content of C18.3n3 and n3 in FF. The last two traits were attributed to better quality oocytes, which contrasts with the high RA of the CTS genes. In conclusion, elevated transcript levels of CTS genes within CC are of limited value as an indicator of reduced quality in bovine oocytes. We suggest that the possible reason for the ambiguity of the obtained data could be the origin of cumulus cells within the COC, which may prove to be crucial for this type of studies.
Authors and Affiliations
Ewelina Warzych, Adam Cieślak, Anna Wolc, Dorota Lechniak
Keywords
Related Articles
- EP ID EP177826
- DOI -
- Views 153
- Downloads 0
QTL in chicken: a look back and forward − a review
Identification of genes determining the expression of economically important traits of plants and animals is a main research focus in agricultural genomics. Most of these traits are characterized by a wide variability of...
HMGR messenger RNA and protein expression in liver, kidney and muscle in pigs of two breeds
Distribution and expression of the 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) in different breeds and tissues of pigs were studied by means of immunohistochemical and RT-PCR methods. Three Huai and three Landrace pi...
Survey of single strand conformation polymorphism of kappa-casein gene in Alpine and Saanen goats in Iran
To study the genetic polymorphism of κ-casein (CSN3) gene as candidate gene for marker-assisted selection in different domestic livestock species, blood samples were randomly withdrawn from 34 Alpine and 42 Saanen goats....
The ostrich meat – an updated review. II. Nutritive value
Ostrich meat is a niche product gaining popularity among consumers especially in Europe. Nutrient composition of this meat considering protein, amino acids, fat, cholesterol, fatty acids, minerals and vitamins was hencef...
The effects of diet supplementation with yeast on the expression of selected immune system genes in the milk somatic cells of dairy goats
The aim of the study was to evaluate the impact of diet supplementation with active yeast culture (Saccharomyces cerevisiae) on the expression of selected immune system genes in goat milk somatic cells (MSC) and on milk...