CAULOGENESIS APPROACHES APPLIED IN VITRO MICROPROPAGATION OF VARIETIES -CABEZA NEGRA 2, ARENA AND RED AMAGER- OF BRASSICA OLERACEA VAR. CAPITATA RUBRA FORM
Journal Title: Current Trends in Natural Sciences - Year 2012, Vol 1, Issue 1
Abstract
In this paper, the authors have proposed a study on the variation in efficiency of micropropagation in vitro due to the influence of donor genotype and explant type used red cabbage head. In this test three commercial varieties of red cabbage seeds were tested for the ability of regeneration by organogenesis from hypocotyls, cotyledons, and stem segments. Our results have shown us that the intensity of red cabbage head regeneration depends on the genotype used but also the type of explant. Hipocotil fragments taken from seedlings to "Cabeza Negra 2"had the greatest total capacity to produce shoots than the other two genotypes tested when the combination of phytohormones was used consisting of 2 mg / l 6-benzylaminopurine (BAP) and type auxine ß indolilacetic acid (IBA) and α naftilacetic acid (NAA) at concentrations of 0.2 to 0.4 mg /l. The inoculated cotyledons explants also developed regenerative callus in about 6-8 weeks after initiation of cultures.
Authors and Affiliations
Silvana Danaila - Guidea, Ana Rosu, Madalina Ionica, Luminita Visan, Ricuta Dobrinoiu
Keywords
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