CCAAT/enhancer binding protein delta (C/EBPδ) deficiency does not affect bleomycin-induced pulmonary fibrosis
Journal Title: Journal of Clinical and Translational Research - Year 2018, Vol 3, Issue 2
Abstract
Background: Idiopathic pulmonary fibrosis is a devastating fibrotic diffuse parenchymal lung disorder that remains refractory to pharmacological therapies. Therefore, novel treatments are urgently required. CCAAT/enhancer binding protein delta (C/EBPδ) is a transcription factor that mediates critical cellular functions in pathophysiology and which was recently suggested to be a key regulatory component in IPF. The purpose of this study was to prove or refute the importance of C/EBPδ in pulmonary fibrosis. Methods: Pulmonary fibrosis was induced by intranasal instillation of bleomycin into wild-type and C/ EBPδ deficient mice. At different time intervals after bleomycin instillation, fibrosis was assessed by hydroxyproline analysis, histochemistry and q-PCR for fibrotic marker expression. Results: C/EBPδ deficient mice developed pulmonary fibrosis to a similar degree as wildtype mice as evident from similar Ashcroft scores, hydroxyproline levels and expression levels of collagen, fibronectin and α-smooth muscle actin at both 14 and 21 days after bleomycin instillation. The resolution of fibrosis, assessed at 48 days after bleomycin instillation, was also similar in wildtype and C/EBPδ deficient mice. In line with the lack of effect of C/EBPδ on fibrosis progression/resolution, macrophage recruitment and/or differentiation were also not different in wildtype or C/EBPδ deficient mice. Conclusions: Overall, C/EBPδ does not seem to affect bleomycin-induced experimental pulmonary fibrosis and we challenge the importance of C/EBPδ in pulmonary fibrosis. Relevance for patients: This study shows that the transcription factor C/EBPδ does not play a major role in the development of pulmonary fibrosis. Pharmacological targeting of C/EBPδ is therefore not likely to have a beneficial effect for patients suffering from pulmonary fibrosis.
Authors and Affiliations
JanWillem Duitman, Cong Lin, Sophie Moog, Madeleine Jaillet, Yves Castier, Aurélie Cazes, Keren S. Borensztajn, Bruno Crestani, C. Arnold Spek
Keywords
Related Articles
- EP ID EP678764
- DOI -
- Views 159
- Downloads 0
Relationship between the Romberg test and the Wii Fit basic balance test and cognition in athletes with concussion
Background: Approximately 30% of individuals with a sport-related concussion present with postural instability. Multiple clinical balance tests exist to diagnose postural instability; yet little is known about the potent...
Effects of prior exercise on glycemic responses following carbohydrate inges on in individuals with type 2 diabetes
Background: Exercise is effective in reducing glycemia, especially when it is performed in the postprandial period. However, no consensus exists in the literature about the effect of exercise on postprandial glucose cont...
Evidence against a role for platelet-derived molecules in liver regeneration after partial hepatectomy in humans
Background and Aim: Blood platelets have been shown to stimulate liver regeneration after partial hepatectomy in animal models and humans, but the molecular mechanisms involved are unclear. It has been proposed that grow...
Social media as a tool to monitor adherence to HIV antiretroviral therapy
Although strict adherence to antiretroviral therapy (ART) lowers morbidity and mortality for people living with HIV and AIDS, there are a number of behavioral, cultural, and psychosocial factors that prevent people from...
Assessment of the Wii Basic Balance Test in measuring postural deficits post-concussion
Background and Aim: To evaluate the Wii Basic Balance Test (WBBT), as a tool for detecting postural instability in athletes with concussions. Methods: Seventy-nine healthy physically active controls (NORM) (mean age 21...