Cell Cycle Proteins (NFkB, P53, P21, P27) Determination in Patients Samples with Hodgkin's Lymphoma
Journal Title: Journal of Medical Science And clinical Research - Year 2014, Vol 2, Issue 6
Abstract
Background: p53 tumor suppressor pathway and other cell cycle regulatory proteins has been suggested as a candidate pathogenic mechanism in Hodgkin's lymphoma ( HL). Aim: evaluating the frequency of of expression of the cell cycle proteins including the mutant p21Ras ,NFkB, p53 and p27 proteins in the malignant Hodgkin/Reed-Sternberg cells ( H/RS cells) of HLsamples and seeking correlation between each protein studied. Methods: A total of 40 archival tissue biopsy samples diagnosed tohave Hodgkin’s lymphoma (HL) were included. The cell cycle regulatory proteins, NFkB, p53, p27 proteins and the mutant p21Ras oncoprotein were studied using insitu hybridization and immunohistochemistry assays.And study the correlation between p21Ras and each of the NFkB, p53 and p27 proteins. Results: The frequency of H/RS cells expression of NFkB, p53, p21 and p27 proteins in HD cases were 17 www.jmscr.igmpublication.org Impact Factor 1.1147 ISSN (e)-2347-176x Nidhal Abdul Mohaimen et al JMSCR Volume 2 Issue 6 June 2014 Page 1548 JMSCR Volume||2||Issue||6||Page 1547-1559||June 2014 2014 (85%), 19 (47%), 29 (72.5) and 9 (22.5%) cases, respectively . The expression was statistically significant [the corresponding Relative Risk (RR) values were 4.52, 5.43, 3.82, and 0.11respectively ]. All cell cycle markers gave nuclear pattern of staining except p21Raswhich gave clear cytoplasmic staining. Correlation between the cell cycle marker revealed no correlation betweenexpression of NFkB and any of the other markers, p53, p21 and p27(p>0.05). p53 exhibited low but insignificant correlation with p21expression (p>0.05), and no correlation with p27, while a highlysignificant but inverse correlation was found between p21 and p27(p<0.01). Conclussion: The high expression of the mutant p21Ras and the low expression of p27 both contribute tocell growth and proliferation albeit through different pathways
Authors and Affiliations
laila Alomer
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