CELLULASE PRODUCING THERMOPHILIC BACTERIA FROM HOT SPRING OF ASSAM
Journal Title: International Journal of Microbiology Research - Year 2016, Vol 8, Issue 8
Abstract
Thirty strains of bacteria were isolated from Garampani, under Nambor reserve forest in Golaghat district of Assam. Qualitative analysis was carried for the thirty isolates, though only eight isolates were found to have cellulose-degrading capacity. Analysis of the 16S rDNA sequences revealed degree of sequence similarity with Bacillus cereus, Bacillus thuringiensis, Stenotrophomonas maltophilia, Bacillus pumilus and Bacillus sp [3]. Growth curve analysis were done at 35°C, 45°C and 55°C. Thermal stability was evaluated at different temperatures ranging from 40°C-100°C as well as the stability of the enzymes were also assessed at different pH range of 2-10. Stenotrophomonas maltophilia (0.43 IU/ml) showed the highest cellulose activity followed by Bacillus cereus (0.39 IU/ml) and Bacillus thuringiensis (0.3 IU/ml) whereas Bacillus cereus (13.77 µg/ml) produced the highest amount of enzyme followed by and Bacillus sp (13.44µg/ml). Highlights • Eight isolates were found to have cellulose-degrading capacity • Bacterial isolates showed high degree of sequence similarity with Bacillus cereus, Bacillus thuringiensis, Stenotrophomonas maltophilia, Bacillus pumilus and Bacillus sp. • Stenotrophomonas maltophilia (0.43 IU/ml) showed the highest cellulase activity • Bacillus cereus (13.77 µg/ml) produced highest amount of enzyme
Authors and Affiliations
ASSMA PARVEEN, ARCHANA DEKA, GUNAJIT GOSWAMI, MADHUMITA BAROOAH, ROBIN CHANDRA BORO
Keywords
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