Change of AOX1a Expression, Encoding Mitochondrial Alternative Oxidase, Influence on the Frost-Resistance of Arabidopsis Plants
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2016, Vol 12, Issue 4
Abstract
The resistance of [i]Arabidopsis thaliana[/i] (L.) Heynh (Columbia ecotype) plants: Col-0 line (wild type), AS-12 line (plants transformed with the construct carrying the [i]AOX1a [/i]gene under control of the CAMV 35S promoter in the antisense orientation) and line XX-2 (plants transformed with the [i]AOX1a [/i]gene construct in the sense orientation) (Umbach et al., 2005), to action of subzero temperature has been studied. It is shown that change of the [i]AOX1a [/i]expression is accompanied by change of the AOX contribution in respiration and increase of the base frost-resistance of Arabidopsis plants. In the leaves of plants with overexpression of [i]АОХ1а [/i]was reduced activity of total superoxide dismutase (SOD), but was increased activity of guaiacol peroxidase and was less content of hydrogen peroxide. It was found that cold hardening during 7 days at 5°C increases the resistance of plants to the subsequent action of subzero temperature regardless of [i]АОХ1а [/i]expression degree. The hardening lead to activation of respiration, increase of the contribution of AOX in the respiration, a significant increase of the water-soluble carbohydrates content and increase of the activity SOD and total guaiacol peroxidases in leaves of all lines the plants. In hardened plants of Arabidopsis wild type and [i]AOX1a[/i] transformants were detected differences in the contents of individual types of reactive oxygen species and the activity of antioxidant enzymes. The trend to decrease of hydrogen peroxide content in lines with altered expression of [i]AOX1a [/i]was observed, but content of superoxide anion radical (SAR) was significantly lower in the AS-12 line compared with the Col-0 and XX-2 plants after hardening. The low content of SAR in leaves of AS-12 line was partly caused by increase of activity total SOD. Thus, we have identified differences in the basic frost-resistance of Arabidopsis plants with altered [i]AOX1a[/i] expression, but significant differences in frost-resistance of hardened plants of wild-type and lines with altered [i]AOX1a [/i]expression was not found. It was concluded that the frost-resistance of plants depends on the activity of AOX, but the decrease of its activity can be compensated by the activation of other protective systems including antioxidant enzymes.
Authors and Affiliations
Olga Grabelnych, Tamara Pobezhimova, Nina Koroleva, Irina Lyubushkina, Natalya Zabanova, Victor Voinikov
Keywords
Related Articles
- EP ID EP101893
- DOI -
- Views 121
- Downloads 0
Change of fatty-acid composition in plants during adaptation to hypothermia
The study of inter-species variability of fatty acid composition in leaf tissues of three species of medicinal plants growing in Predbaikal’ye showed that this composition has a species-specific character. In the composi...
AGROBACTERIAL TRANSFORMATION AS COMPLEX BIOTICAL STRESSING FACTOR
Consequences of agrobacterial transformation have resemblance with plant stress response. So agrobacterial transformation is being considered as a complex multilevel biotic stress factor including reactions on the wound...
Synergetic action of light and temperature on seed germination of some solanaceae members
The objective of the present study was to evaluate the effects of nine combinations of temperature and light on seeds germination of four solanaceae species ([i]Datura innoxia, Hyoscyamus muticu...
Body mass, Thermogenesis and energy metabolism in Tupaia belangeri during cold acclimation
In order to study the relationship between energy strategies and environmental temperature, basal metabolic rate (BMR), nonshivering thermogenesis (NST), the total protein contents, mitochondrial protein contents, state...
Role of Nitric oxide in regulation of H[sub]2[/sub]O[sub]2[/sub] mediating tolerance of plants to abiotic stress: A synergistic signaling approach
The relationship between abiotic stress, nitric oxide (NO) and Hydrogen peroxide (H[sub]2[/sub]O[sub]2[/sub]) is a challenging one. It is now clear that H[sub]2[/sub]O[sub]2[/sub] and NO function as signaling molecules i...