Change of AOX1a Expression, Encoding Mitochondrial Alternative Oxidase, Influence on the Frost-Resistance of Arabidopsis Plants
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2016, Vol 12, Issue 4
Abstract
The resistance of [i]Arabidopsis thaliana[/i] (L.) Heynh (Columbia ecotype) plants: Col-0 line (wild type), AS-12 line (plants transformed with the construct carrying the [i]AOX1a [/i]gene under control of the CAMV 35S promoter in the antisense orientation) and line XX-2 (plants transformed with the [i]AOX1a [/i]gene construct in the sense orientation) (Umbach et al., 2005), to action of subzero temperature has been studied. It is shown that change of the [i]AOX1a [/i]expression is accompanied by change of the AOX contribution in respiration and increase of the base frost-resistance of Arabidopsis plants. In the leaves of plants with overexpression of [i]АОХ1а [/i]was reduced activity of total superoxide dismutase (SOD), but was increased activity of guaiacol peroxidase and was less content of hydrogen peroxide. It was found that cold hardening during 7 days at 5°C increases the resistance of plants to the subsequent action of subzero temperature regardless of [i]АОХ1а [/i]expression degree. The hardening lead to activation of respiration, increase of the contribution of AOX in the respiration, a significant increase of the water-soluble carbohydrates content and increase of the activity SOD and total guaiacol peroxidases in leaves of all lines the plants. In hardened plants of Arabidopsis wild type and [i]AOX1a[/i] transformants were detected differences in the contents of individual types of reactive oxygen species and the activity of antioxidant enzymes. The trend to decrease of hydrogen peroxide content in lines with altered expression of [i]AOX1a [/i]was observed, but content of superoxide anion radical (SAR) was significantly lower in the AS-12 line compared with the Col-0 and XX-2 plants after hardening. The low content of SAR in leaves of AS-12 line was partly caused by increase of activity total SOD. Thus, we have identified differences in the basic frost-resistance of Arabidopsis plants with altered [i]AOX1a[/i] expression, but significant differences in frost-resistance of hardened plants of wild-type and lines with altered [i]AOX1a [/i]expression was not found. It was concluded that the frost-resistance of plants depends on the activity of AOX, but the decrease of its activity can be compensated by the activation of other protective systems including antioxidant enzymes.
Authors and Affiliations
Olga Grabelnych, Tamara Pobezhimova, Nina Koroleva, Irina Lyubushkina, Natalya Zabanova, Victor Voinikov
Keywords
Related Articles
- EP ID EP101893
- DOI -
- Views 108
- Downloads 0
The Effect of Osmo and Hormone Priming on Germination and Seed Reserve Utilization of Millet Seeds under Drought Stress
The objective of this research was to evaluate the effect of seed priming with osmo and hormone priming on growth and seed reserve utilization of millet seeds under drought stress. Treatments were combinations of 4 level...
BIOTIC STRESS IMPACT ON ACTIVITY OF VARIOUS FORMS OF ADENYLATE CYCLASE IN ORGANELLES OF POTATO PLANT CELLS
Notwithstanding significant interest towards study of adenylate cyclase plant signal system, there is still no complete picture of functioning and regulation mechanisms of this signal system in plants under biotic stress...
GENETICS AND GENOMICS OF PLANT GENETIC RESOURCES
A comparative study of lichen biochemistry and air pollution status of urban, semi urban and industrial area of Hooghly and Burdwan district, West Bengal
Toxicity Concerns of Semiconducting Nanostructures on Aquatic Plant Hydrilla verticillata
In this article, we have examined toxicity of nanostructures such as flower-like ZnO capped with starch, spherical uncapped ZnO and spherical CdS on aquatic plant Hydrilla verticillata, which has not done before. Hydrill...