Changes in intrinsic excitability of ganglion cells in degenerated retinas of RCS rats
Journal Title: International Journal of Ophthalmology - Year 2018, Vol 11, Issue 5
Abstract
"AIM: To evaluate the intrinsic excitability of retinal ganglion cells (RGCs) in degenerated retinas. METHODS: The intrinsic excitability of various morphologically defined RGC types using a combination of patch-clamp recording and the Lucifer yellow tracer in retinal whole-mount preparations harvested from Royal College of Surgeons (RCS) rats, a common retinitis pigmentosa (RP) model, in a relatively late stage of retinal degeneration (P90) were investigated. Several parameters of RGC morphologies and action potentials (APs) were measured and compared to those of non-dystrophic control rats, including dendritic stratification, dendritic field diameter, peak amplitude, half width, resting membrane potential, AP threshold, depolarization to threshold, and firing rates. RESULTS: Compared with non-dystrophic control RGCs, more depolarizations were required to reach the AP threshold in RCS RGCs with low spontaneous spike rates and in RCS OFF cells (especially A2o cells), and RCS RGCs maintained their dendritic morphologies, resting membrane potentials and capabilities to generate APs. CONCLUSION: RGCs are relatively well preserved morphologically and functionally, and some cells are more susceptible to decreased excitability during retinal degeneration. These findings provide valuable considerations for optimizing RP therapeutic strategies."
Authors and Affiliations
Cong-Jian Zhao
Keywords
Related Articles
- EP ID EP489832
- DOI 10.18240/ijo.2018.05.07
- Views 76
- Downloads 0
Predictive factors of visual outcome of Malaysian cataract patients: a retrospective study
"AIM: To explore the associations between various characteristics of Malaysian cataract patients and their management, and their post-operative visual outcomes, to inform relevant bodies to reduce cataract-related blindn...
Changes in intrinsic excitability of ganglion cells in degenerated retinas of RCS rats
"AIM: To evaluate the intrinsic excitability of retinal ganglion cells (RGCs) in degenerated retinas. METHODS: The intrinsic excitability of various morphologically defined RGC types using a combination of patch-clamp re...
The prognostic value of lymphocyte-to-monocyte ratio in retinopathy of prematurity
"AIM: To evaluate the associations between development of retinopathy of prematurity (ROP) and serum lymphocyte-to-monocyte ratio (LMR), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR). METHO...
Comparison of visual performance recovery after thin-flap LASIK with 4 femtosecond lasers
"AIM: To compare the speed of visual recovery following myopic thin-flap LASIK with four femtosecond lasers. METHODS: Eighty-eight eyes of 46 patients who were consecutively scheduled for bilateral LASIK with the IntraLa...
Comparison of peripapillary and macular choroidal thickness and ganglion cell complex thickness in glaucomatous and healthy eyes
"AIM: To assess choroidal thickness (CT) and its association with ganglion cell-inner plexiform layer thickness (GCIPLT) and retinal nerve fiber layer thickness (RNFLT) in open angle glaucoma (OAG) comparing with preperi...