Changes in proteins and tenderness of meat from young bulls of four breeds at three ages over 10 days of cold storage
Journal Title: Animal Science Papers and Reports - Year 2010, Vol 28, Issue 1
Abstract
The study aimed at analysing the changes taking place in the muscle tissue of cattle during meat cold storage in relation to animal genotype (breed) and age. Investigations were conducted on the thoracic and lumbar part of the longissimus dorsi (LD) muscle of Polish Holstein-Friesian (PHF) Black-and-White variety, Polish Red (PR), Hereford (H) and Limousine (L) bulls slaughtered at the age of 6, 9 and 12 months. Muscle analyses were carried out 45 min and 48, 96 and 240 h post- slaughter, separating proteins with the assistance of SDS-PAGE (electrophoresis in polyacrylamide gel using SDS). To identify titin, desmin and troponin T (Tn-T), their antibodies and western blotting were employed. The breed occurred to be the key factor affecting proteins’ changes in the muscle tissue. The process of protein degradation in PHF was similar to that found in H while in PR – to that occurring in L bulls, despite the genotype differences between them. The greatest differences in protein changes were found between the meat obtained from bulls at the age of 6 vs . 12 months. During meat cold storage, day 10 turned out to be critical with regard to the degradation of almost all proteins. The drop of the content of titin, desmin and Tn-T was observed, simultaneously with the increase in their degradation products. The highest myofibrillar protein degradation observed on day 10 of cold storage proves these changes.
Authors and Affiliations
Agnieszka Iwanowska, Ewa Iwañska, Bożena Grześ, Beata Mikołajczak, Edward Pospiech, Stanisław Rosochacki, Edyta Juszczuk-Kubiak, Andrzej Łyczyński
Keywords
Related Articles
- EP ID EP71395
- DOI -
- Views 107
- Downloads 0
Genotype-environment interaction between Chile and North America and between Chilean herd environmental categories for milk yield traits in Black and White cattle
In order to evaluate the expression of sire genetic evaluations obtained in North America for Chilean Black and White Cattle, genetic correlations were estimated for milk (MY), fat (FY) and protein (PY) yields, and for f...
Efficacy of free-choice feeding of ground wheat or whole grain wheat and protein concentrate to turkeys
The aim of this study was to determine body weight gains, feed intake and feed consumption patterns in turkeys, when offered a choice of various feed ingredients (protein-fat-mineral-vitamin concentrate pellets, ground-p...
Changes in proteins and tenderness of meat from young bulls of four breeds at three ages over 10 days of cold storage
The study aimed at analysing the changes taking place in the muscle tissue of cattle during meat cold storage in relation to animal genotype (breed) and age. Investigations were conducted on the thoracic and lumbar par...
Effect of sex, muscle, and processing temperature on heme iron content in lamb meat
The objectives of this study were to assess the effect of sex and a muscle type on heme iron content in meat of Uhruska lambs, and to determine the effect of different processing temperatures (60, 70, 80, and 90°C) on co...
Factors affecting genetic correlation estimates from dairy sires’ genetic evaluations to assess genotype-environment interaction
Effects of trait heritability (0.05 or 0.25), effective daughters number (30 to 500), sires number (10 to 500), and sire selection (selecting or not the top 50% sires), were evaluated based upon standard error (SE) and b...