Changes in Proteins in Aqueous Humor of Glaucoma Patients Compared to Cataract Patients
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 19, Issue 2
Abstract
Purpose: The Aqueos Humour (AH) is well accessible and provides nutrition and immunity in the anterior segment of the eye. Purpose of our study was to uncover glaucoma related proteomic changes in AH. Patients and Methods: AH samples from 35 patients (Controls: n=12, age=67.4±13.6; POAG: n=23, age: 72.5±8.3) were obtained during cataract or glaucoma surgery. AH samples were subsequently pooled into the experimental groups under equal contribution in terms of protein amount of each individual patient. Protein samples were analysed by LTQ Orbitrap MS device with an upstream LC system. The obtained raw data were analysed using the Maxquant proteome software. Proteins with a fold-change ratio up-regulation higher than a cut-off of 2 were considered as noticeably altered. Results: Quantitative mass spectrometric analysis of AH from POAG- and cataractpatients identified a total number of 175 proteins. Apolipoprotein D (3.16), complement C3 (2.96) pigment epithelium-derived factor (fold change 2.86), dickkopf-related protein 3 (fold change 2.18), Wnt inhibitory factor 1(fold change 2.35) were significantly changed within the AH of glaucoma patients.Glaucoma is a leading cause for vision loss and blindness worldwide. Glaucoma is characterized by accelerated Retinal Ganglion Cell (RGC) and axonal degeneration, which results in progressing irreversible visual loss and ultimately blindness in patients. Elevated Intraocular Pressure (IOP) is the main risk factor and controlling IOP is by far the only efficient treatment in clinical practice [1]. The stabilization of IOP is majorly depend on the balance of production and drainage of Aqueous Humour (AH). The accumulation of excessive AH amounts leads to increased IOP. Besides this, AH also provides nutrition and immunity in the anterior segment of the eye. Its protein composition alters depending on various ocular conditions. Even though, the exact pathogenesis of glaucoma remains obscure, the change in AH protein may provide an insight into involved molecular mechanisms of glaucoma, and its early diagnosis, prediction of prognosis, and patients’ responses to treatments. Proteomics as a rising technique for biomarker discovery, is used in this study to uncover glaucoma related proteomic changes in AH. Purpose of our study was to uncover glaucoma related proteomic changes in AH.
Authors and Affiliations
Hanhan Liu, Fabian Anders, Sebastian Funke, Norbert Pfeiffer, Franz Grus, Verena Prokosch
Keywords
Related Articles
- EP ID EP622657
- DOI 10.26717/BJSTR.2019.19.003273
- Views 158
- Downloads 0
An Unusual Case of Statin-Related Myopathy
A 68 year male patient (KB) presented in August 2014 with profound muscle weakness of the hip and legs. Treatment with an 8 cycle regimen of CHOP for NHL 10 years earlier led to complete remission. He had also been treat...
Reconstruction of a Worn or Mutilated Dentition with the Utilization of an over Denture Appliance for a Patient with Enamel Deficiency - A Case Report
Background: Evidence suggests that the retention of mandibular roots for the preservation of bone in patients by utilizing an over denture is a conservative approach for a fixed and removable prosthetic reconstruction. C...
Atypical Granular Cell Tumour of the Vulva
Granular cell tumours are benign tumours that are thought to originate from the Schwann cells. They tend to occur in middle aged adults and can be found anywhere in the body in subcutaneous as well as dermis tissue [1]....
Herbal Extracts Used in Dental Disorders
Natural products have been recently investigated more thoroughly as promising agents for the prevention of oral diseases, especially plaque-related diseases such as dental caries. Oral diseases, a major health issue in t...
Ascitic Type of Abdominal Tuberculosis in a 40 Year Old Female
Disseminated tuberculosis can cause a lot of damage to an individual if there is no early diagnosis. In Africa, about 70% of patients with abdominal tuberculosis present with ascites and most of these cases are missed di...