Changes in serum immunoglobulin levels in children with thalassemia who undergo repeated blood transfusions and their correlation with delayed hemolytic transfusion reactions
Journal Title: Chinese Journal of Blood Transfusion - Year 2024, Vol 37, Issue 5
Abstract
Objective To study the changes in serum immunoglobulin levels in children with thalassemia who undergo repeated blood transfusions and explore their correlation with delayed hemolytic transfusion reactions(DHTR). Methods Serum samples from children with thalassemia who received blood transfusion treatment from June 2022 to April 2023 (observation group) and healthy children who underwent physical examination (control group) in our hospital were collected. The levels of serum immunoglobulins (IgG subtype, IgM, IgA, IgE and IgD) were detected using flow cytometry CBA multi-factor quantitative detection technology, and the differences between the two groups were compared. The children were divided into 4 groups according to different transfusion numbers: ≤10 numbers, 11-30 numbers, 31-50 numbers and >50 numbers, and the differences between different blood transfusion numbers and serum immunoglobulin levels in each group were compared using one-way analysis of variance (ANOVA). Children with thalassemia with DHTR were in the hemolysis group, and children with thalassemia who did not experience DHTR were in the non-hemolysis group. The changes in serum immunoglobulins (IgG subtypes, IgM, IgA, IgE and IgD) between the two groups were compared to explore the correlation between serum immunoglobulins in thalassemia children with repeated transfusion and DHTR. Results The levels of IgG1, IgG3, IgG4 and IgA in the observation group were significantly higher than those in the control group, with the increase of(2.07±2.12), (0.67±2.03), (0.30±0.37)and(6.04±11.40)mg/mL, respectively, while the level of IgD in observation group was significantly lower than that in the control group, with a decrease of(0.03±0.01)mg/mL, P<0.05. No significant difference was noticed in IgG2, IgM and IgE between the groups(P>0.05). IgG1 and IgG4 both significantly increased with the number of blood transfusions.The IgG1 in the 4 groups increased sequentially as(0.30±0.62), (0.41±0.51)and(3.60±3.48)mg/mL, and IgG4 increased sequentially as (0.12±0.13), (0.22±0.07) and (0.21±0.38)mg/mL. IgG2, IgM and IgD showed a significant decrease, with IgG 2, IgM, and IgD in four groups decreased as(0.91±1.50), (0.14±0.10)and(0.05±0.05)mg/mL, respectively, showing significant differences with the number of blood transfusions(P<0.05). No significant difference was found in IgG3, IgA and IgE with different number of transfusions(P>0.05). IgG1, IgG3 and IgG4 in the hemolysis group were significantly higher than those in the non-hemolysis group, with an increase of (4.44±3.41), (0.73±1.26)and(0.52±0.40), respectively(P<0.05).IgD in the hemolysis group was significantly lower than that in the non-hemolysis group, with a decrease of (0.00±0.06)mg/mL, P<0.05. No significance was noticed in IgG2, IgM, IgA and IgE between the hemolysis group and the non-hemolysis group(P>0.05). Conclusion The serum immunoglobulin levels of children with thalassemia who undergo repeated blood transfusions are abnormal. There are differences in correlation between the number of blood transfusions and serum immunoglobulin levels among children with thalassemia who undergo repeated blood transfusions. The relevant serum immunoglobulins for DHTR in children with thalassemia who undergo repeated blood transfusions are IgG1, IgG3 and IgG4.
Authors and Affiliations
Xiaohong JIN, Meikun HU, Rui CHEN, Lilan GAO, Shuxia WANG, Mengxing LYU, Kexuan QU
Keywords
Related Articles
- EP ID EP738819
- DOI 10.13303/j.cjbt.issn.1004-549x.2024.05.010
- Views 4
- Downloads 0
Determination of the ELISA kits for screening convalescence plasma with high potency of SARS-CoV-2 IgG
Objective To determine the ELISA kit for screening convalescence plasma with high potency of SARS-CoV-2 IgG by comparing and analyzing the plasma detection results of convalescent plasma collected in different periods vi...
Meta analysis of the effect of pre-transfusion prophylactic medication on the incidence of adverse reactions to blood transfusion
Objective To systematically evaluate the correlation between pretransfusion prophylactic medication and the incidence of adverse reactions to blood transfusion(ARBT) by Meta analysis. Methods The relevant literature conc...
Molecular genetic analysis of a patient with double population of red blood cells for RhD antigen
Objective To solve the difficulty of RhD blood group typing in a patient with double population(DP) of red blood cells for RhD antigen by serological and genotyping analysis. Methods Separation of the two populations of...
Effect of high intraoperative plasma dose on the prognosis of patients undergoing blood transfusion during perioperative period
Objective To explore the relationship between the intraoperative plasma transfusion volume, the changes of blood coagulation test values and the clinical prognosis of surgical patients, so as to provide a theoretical bas...
Proteomic characterization of platelet-derived extracellular vesicles induced by different activation pathways
Activated platelets secrete bioactive molecules and extracellular vesicles. Platelet-derived extracellular vesicles(PEVs), which are rich in proteins, have become important mediators of intercellular communication and ca...