CHANGING OF SOME STENOTROPHOMONAS MALTOPHILIA BIOLOGYCAL PROPERTIES UNDER EFFECT OF SODIUM DODECYL SULFATE
Journal Title: Мікробіологія і біотехнологія - Year 2014, Vol 0, Issue 1
Abstract
Aim: To study biological abilities changing of Stenotrophomonas maltophilia before and after cultivation on the medium with sodium dodecyl sulfate (SDS). Methods. The physiological and biochemical properties, antibiotics sensitivity of S maltophilia were studied due to microbiological analyzer Vitek 2 Compact (Biomerieus, France), cells morphology – light microscopy method (Zeiss Primo Star, Germany) and electron microscopy (Jeol 1400, Japan). Results. The short-time cultivation of S maltophilia (during 1–10 running) in presence of synthetic anionic surfactant SDS in concentration of 50 mg/L that is near minimal inhibition concentration (MIC) results in changing of some biological parameters and properties, e.g. cells became shorter and changed their shape, the different optical density zone were noted in cytoplasm, the dark and transparent inclusion appeared in cells. Some biochemical test results (glutamyl arylamidase, tyrosine arylamidase, urease, citrate (sodium), beta-N-acetyl-galactosidase, L-histidine assimilation, coumarate, 0/129 resistance, L-malate assimilation, Ellman) were changed to opposite index. The MIC of 9 antibiotics increased in 2 times and more, to 4 antibiotics there were formed resistance. Adhesion properties of S. maltophilia varied depending upon run times in presence of SDS. The properties which were changed during the experiment sometimes were restored after the 10th run. This fact could speculate about certain culture adaptation toward to antibiotic. Whereas SDS is the most popular surfactant, which is included in almost all cosmetic and household chemicals, the changes of biological activities of bacteria under its influence could provoke the problems with clinical important strains identification.
Authors and Affiliations
O. Radchenko, L. Stepura, Iu. Iumyna, M. Boretska, P. Zelena
Keywords
Related Articles
- EP ID EP395249
- DOI 10.18524/2307-4663.2014.1(25).48204
- Views 115
- Downloads 0
BIOLOGICAL ACTIVITY OF RH IZOSPHERE SOIL SPRING WHEAT IN ASSOCIATION WITH BACTERIA AZOTOBACTER CHROOCOCCUMKZT79. MODIFIED N-ACETYL-D-GLUCOSAMINE
Aim. The study of the biological activity of rhizosphere soil of the plants of spring wheat in association with bacteria Azotobacter chroococcum T79, modified N-acetyl-D-glucosamine. Methods. The biological activity of t...
SCREENING OF SIDEROPHORE PRODUCERS AMONG PANTOEA AGGLOMERANS STRAINS
Aim. Screening of siderophore-producing strains of P. agglomerans. Methods. The siderophore-producing ability of P. agglomerans strains have been investigated by using of chrome azurol S (CAS) assay. The ternary complex...
EFFECT OF MULTIPROBIOTIC «SYMBITER ACIDOPHILIC» USED AGAINST THE BACKGROUND OF THE COURSE OF INJECTIONS OF CEFTRIAXONE ANTIBIOTIC ON PERITONEAL MACROPHAGE FUNCTIONAL ACTIVITY IN RAT
The aim of the work was to investigate the effect of probiotic preparation “Symbiter acidophilic” used against the background of the course of injection of ceftriaxone antibiotic on macrophage functional activity in Wist...
ESTIMATION OF PRODUCTIVITY BACILLUS THURINGIENSIS ON DIFFERENT MEDIA
Aim: Studying the nutrient medium influence on productivity and insecticidal activity of entomopathogenic bacteria Bacillus thuringiensis (Bt). Materials and methods. The reference exotoxin strain B. thuringiensis var. t...
FATTY ACID COMPOSITION OF LIPIDS OF BACTERIA OF THE GENUS PSEUDOMONAS, OXIDIZING PETROLEUM PRODUCTS
Aim. Determination of fatty acid composition of lipids and identification of strains of bacteria of the genus Pseudomonas, which oxidize crude oil and petroleum products. Methods. The fatty acid analysis of strains of Ps...