Characteristics of Ethanol Extract of Detam 1 Indonesian Soybean and Jati Belanda Leaves and the Effects of their Combinations on Weight Gain and Jejunum Histopathological Changes in Male Wistar Rats
Journal Title: European Journal of Medicinal Plants - Year 2015, Vol 7, Issue 2
Abstract
Background: Anti-Nutritional-Factors (ANF) are natural or synthetic compounds that interfere with the absorption of nutrients. ANF may cause changes in the histopathology of the small intestinal mucosa, and may affect the digestive, secretory and absorptive functions of the small intestine. Soybean and Jati Belanda leaves which can promote weight loss, may contain ANF. In this study, soybean seeds variety Detam 1 and Jati Belanda leaves were used to assess their potential effects on weight gains and jejunum functions. Research Objective: To study the effects of ethanol extracts of Detam 1 raw soybean (EEDS) and Jati Belanda (EEJB) Bumi Herbal Dago variety and their combinations on weight gain and jejunum histopathological changes in male Wistar rats. Research Methods: Phytochemical analyses of EEDS and EEJB ethanol extracts were performed, then assayed for the above mentioned activities after administered to high-fat induced male Wistar rats for 28 days. Weight gain was determined and histopathological examination of jejunum was achieved after fixation and staining with Hematoxylin Eosin. Results: The combinations of EEDS:EEJB (2:1 and 1:2 w/w) reduced the weight gain with the effect being more pronounced (5.95%) for the combination 1:2 (w/w). Such effect may be attributed to ANFs contained in EEDS and EEJB such as tannin, saponin, flavonoids and phenolics. The histopathologic examination of the positive control group was not different from all treatment groups (p>0.05). Conclusion: The combination EEDS:EEJB (1:2 w/w) is effective in inhibiting weight gain without remarkable histopathological changes in male Wistar rat jejunum.
Authors and Affiliations
Meilinah Hidayat, Sylvia Soeng, Sijani Prahastuti, Hartini Tiono, K. Yonathan Ari, Monica Sugiono
Keywords
Related Articles
- EP ID EP350166
- DOI 10.9734/EJMP/2015/13440
- Views 174
- Downloads 0
Aqueous Extract of the Fruits of Xylopia aethiopica (Dunal) A. Rich. Protects against Carbon Tetrachloride - Induced Hepatotoxicity in Rats
Aim: This study was designed to examine the hepatoprotective effect of aqueous extract of the fruits of Xylopia aethiopica using the carbon tetrachloride (CCl4) model. Place and Duration of Study: Department of Chemical...
In vitro Evaluations of Cytotoxicity and Anti-inflammatory Effects of Peganum harmala Seed Extracts in THP-1-derived Macrophages
Aim: Based on knowledge from traditional Greco-Arab herbal medicine, this in vitro study aims to evaluate the cytotoxicity and the anti-inflammatory mechanism of Peganum harmala (P. harmala) seeds by measuring the expres...
Microtuberization of Ceropegia pusilla Wight and Arn. an Endangered Medicinal Plant
An efficient micropropagation (direct & indirect) and microtuberization protocol for an endemic and ethanomedicinal plant Ceropegia pusilla is described. The highest number of shoot initiation (3.50 0.34) and higher ave...
Protective Effects of Zanthoxylum chalybeum in Diabetes-induced Myocardial Dysfunction in Rats
Background: Control of glycaemia and hyperlipidemia are important objectives in preventing diabetes induced cardiac dysfunction. Objective: The aim of the study was to examine the effects of Zanthoxylum chalybeum in myoc...
Neuropharmacological Activities of Ethanolic Extract of Cola millenii Dried Leaf in Rats
Objective: The leaf of Cola millenii is used in ethnomedicine to treat several ailments including; infections, fever and pain among several other diseases in Southwest Nigeria. Preliminary report indicates that the ethan...