Characterization and Invitro Cytotoxicity of T-2 Toxin Isolated from Corn

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Characterization and Invitro Cytotoxicity of T-2 Toxin Isolated from Corn

Journal Title: International Journal of Current Research and Review - Year 2018, Vol 10, Issue 1

Abstract

Aim: Main objective of the study is to characterize and evaluate invitro cytotoxicity of T-2 toxin isolated from corn. Methodology: The fungal strain was isolated by standard blotter method; morphological identification was done using scanning electron microscope (SEM). The fungal strain was identified as F. sporotrichioides based on its cultural and morphological characteristics and internal transcribed spacer (ITS/ITS4) primers. The toxin was characterized using thin layer chromatography (TLC), HPLC and Fourier Transform Infrared Spectroscopy (FTIR) Result: 100ml of culture filtrate yielded 10.126µg of toxin. The functional groups of toxin were identified by (FTIR) method. Invitro cytotoxicity of T-2 toxin was evaluated on human keratinocytes, hepatoma and neuron cell lines. HaCaT and HepG2 cells were found to be more sensitive than SHSY5Y to T-2 toxin exposure at 50-100ng and 100-200ng. Discussion and conclusion: T-2 toxin was extracted and characterized from corn samples, by analytical and molecular methods in addition FTIR analysis verifies the functional groups of T-2 toxin. Further invitro cytotoxicity was evaluated using different human cell lines. Comparably skin and liver cell lines found more sensitive to T-2 toxin exposure. Sensitivity of the cell lines to the toxin at ng may be due to the purity of the toxin

Authors and Affiliations

Dr. Farhath Khanum

Keywords

EP ID EP533253
DOI 10.7324/IJCRR.2018.1019
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