Characterization of antimicrobial resistant bacterial pathogens recovered from cases of bovine mastitis with special reference to Staphylococcus aureus
Journal Title: Journal of Veterinary Medical Research - Year 2016, Vol 23, Issue 1
Abstract
In the current study, a total of 20 and 78 milk samples were collected from animals showed signs of clinical and subclinical mastitis, for isolation and identification of different causative pathogens in some dairy farms of Beni-Suef Governorate, and for investigation of in vitro sensitivity. The recovered microorganisms were Staphylococcus species (n=79; 80.61%), Enterococcus spp. (n=28; 28.57%), CAMP negative Streptococci, Pseudomonas aeruginosa (n=7; 7.14%), E. coli (n=3; 3.06%) and Proteus vulgaris (n=1; 1.02%). Antibiogram profile for S. aureus showed that the most effective drug was vancomycin and the least was penicillin. Trials were done to detect biofilm production for recovered isolates of S. aureus (n=23) by the use of a phenotypic method (Congo red agar, CRA) and genotypic methods through determination of some biofilm related genes using PCR. All recovered S. aureus isolates were seeded on the CRA media to detect the biofilm forming ability. It has been found that all tested isolates showed a biofilm forming ability either strong (13; 56.52%) or intermediate (10; 43.48%). The detection of some biofilm associated genes (icaA, icaD and bap genes) using polymerase chain reaction revealed that two (10.53%) isolates out of 19 were negative for all tested genes, 16 (84.21%) isolates harbored both icaA and icaD gene, while only one (5.26%) isolate had all tested genes.
Authors and Affiliations
Hassan W. H. , Hatem M. E. , Elnwary H. A. , Sediek S. H.
Keywords
Related Articles
- EP ID EP658377
- DOI -
- Views 221
- Downloads 0
Prevalence of udder and teat affections in large ruminant in Beni-Suef and El-Fayoum provinces
This investigation was carried out on 5457 animals; among which, 3916 cows, 1531 buffaloes and 10 she-camels in Beni-Suef and Fayoum provinces. Animals were subjected to clinical examination to study the congenital and a...
Some pharmacodynamic interactions between salinomycin and vitamin E or selenium in chickens
The present study was conducted to determine the effect of salinomycin at a concentration 60 and 120 ppm alone and with vitamin E or selenium on haematological and biochemical parameters and histopathological changes of...
Attenuation effect of UV on Haemonchus contortus Larvae in Experimentally Infected Goats
This study was conducted to study the UV protective effect against Haemonchus contortus infection in goats. Sixteen male goats were divided into 5 groups, control infected, control uninfected and UV 30minutes; UV 60minut...
Pharmacokinetic profile and some pharmacodynamic aspects of cefquinome in chickens
The pharmacokinetic profile and some pharmacodynamic aspects of cefquinome were studied after intramuscular (IM) and subcutaneous (SC) administration of a single dose of 2 mg kg-1 b.wt. in chickens. Tissue distribution a...
Physicochemical and bacteriological status of retail-marketed shrimps and crabs in Beni-Suef, Egypt
A total of a hundred and twenty samples of crustaceans, 60 samples of shrimp (30 peeled & 30 unpeeled shrimps) and 60 samples of local chilled crab were collected from Beni-Suef markets. Collected samples were evaluated...