Characterization of Group B Streptococci from Rural Women Attending Primary Health Centre
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH IN MEDICAL SCIENCES - Year 2016, Vol 2, Issue 1
Abstract
The purpose of this study was to screen both pregnant and non-pregnant women in rural areas for GBS. A total of 30 vaginal samples were collected from patients attending Primary Health Care Centre, Cuddalore. The samples were immediately transported to BHI broth in an ice pack and taken to the laboratory. Samples were inoculated into 5% blood agar plates, incubated at 37˚C in an anaerobic jar for overnight. Both hemolytic and non-hemolytic colonies were identified by standard biochemical tests, Anindita Das et al., (2003). The grouping was done by micro nitrous acid extraction, co-agglutination technique. Antibiogram was performed b y Kirby Bauer Disc Diffusion method. Similarly, 30 cervical were analyzed by direct plating. Out of the 15 samples, from non-pregnant women 26.67% (4) were positive out of 15 samples from pregnant women 13.33% (2) were positive for GBS. 40% and 33.33% were positive for Staphylococcus species and Gram negative rods respectively from non-pregnant women. 46.67% and 40% were positive for Staphylococcus species and Gram-negative rods respectively from pregnant women, Badri et al., (1997). All the six isolates were resistant to antibiotics like Erythromycin, Neomycin and Tetracycline and were sensitive to other antibiotics like Cephotaxime, Penicillin G and Ampicillin. Therefore screening for GBS carriers in non-pregnant women is necessary, Berkowitz et al., (1990).
Authors and Affiliations
A. Balasubramanian* S. Devi
Keywords
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