Characterization of Pathogenic Bacteria from Raw and Pasteurized Milk of Different location of Dhaka city in Bangladesh
Journal Title: Scholars Academic Journal of Biosciences - Year 2016, Vol 4, Issue 5
Abstract
Abstract:The aim of study was to characterize the bacteriological quality of locally available raw milk and pasteurized milk. A total number of thirty two raw milk and pasteurized samples were collected from Dhaka city and its surrounding areas. For the determination of sanitary, total viable bacterial count (TVBC), total coliform count (TCC) and total staphylococcal count (TSC) analysis was performed. The highest TVBC, TCC and TSC were 6.8 x 105 cfu/ml, 85cfu/ml & 4.5x105 cfu/ml, respectively. In order to observe the morphological characteristics, biochemical characteristics and milk quality, gram staning, MR, VP, Indole, Citrate, Urease, Catalase, Oxidase and Starch hydrolysis was carried out. All the sample from raw and pasteurized milk gave starch hydrolysis, oxidase and indole negative result but catalase positive. Indicating the presence of Shigella dysenteriae, Klebsiella pneumonia, Salmonella spp. Proteus spp. Klebsiella spp. Salmonella spp, E.coli Pseudomonas spp. This survey indicates that most of the raw milk samples were not satisfactory in course of public health standard as some pathogenic bacteria were detected from these samples. Proper processing regarding pasteurization recommended to milk samples. Attention must be paid to save the raw milk as well as the pasteurized milk from the thread of milk quality related problems and public health concern. Keywords:Pasteurization, raw milk, Staphylococcus, E. coli and Salmonella species.
Authors and Affiliations
Safirun Pervin, Aeyasa Akter, Muhammad E. Uddin, Tanzina Akter
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