Characterization of PSB Isolates in Rhizosphere Soil of Rice Varieties Grown at OFRC, SKUAST Jammu
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2018, Vol 7, Issue 9
Abstract
Phosphate is the key macronutrient required for the growth of plants. The soluble forms of phosphorus when applied to soil are rendered insoluble by undergoing chemical fixation. The soil collected from organic farm, SKUAST- Jammu was investigated for screening the phosphate solubilizing bacteria. In the present study, soil samples were collected from rice rhizosphere of sixteen varieties grown in OFRC, SKUAST- Jammu. This study revealed the existence of Phosphate Solublizing bacteria in the rhizosphere soils of rice. These bacteria exhibited difference with respect to Gram property, nitrate reductase, oxidase, citrate utilization and catalase activities. Prominent halo zones were found in case of positive PSB isolates on Pikovskaya’s agar. Phosphate solubilization effectively took on sixth day. Based on Gram property, bacterial morphology and biochemical tests performed on various isolates, these were subjected to identification and placed in group I and VIII by referring to the separation outlined in Bergeys Manual of Systematic Bacteriology and confirms the dominance of genus Bacillus and Pseudomonas. The identification at species level will be further undertaken using 16S rRNA technique.
Authors and Affiliations
Renu Gupta, J. P. Sharma
Keywords
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- EP ID EP582386
- DOI 10.20546/ijcmas.2018.709.001
- Views 52
- Downloads 0
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