Characterization of the mitochondrial genome and phylogenetic analysis of Sorita pulchella
Journal Title: Journal of Henan Agricultural University - Year 2023, Vol 57, Issue 2
Abstract
[Objective] The objective of this study was to investigate the characteristics of mitochondri-al genome of Sorita pulchella, to explore its taxonomic status, and to analyze the phylogenetic relationship of higher taxonomic category of moths. [Method] The samples of S. pulchella was gathered in the Yigong Tea Farm in Nyingchi City, Tibet Autonomous Region. High-throughput sequencing was used to obtain the mitochondrial genome sequence of S. pulchella, and its genome characteristics were analyzed. Maximum Likelihood and Bayesian inference methods were used to establish phylogenetic tree to explore the phylogenetic relationships among moths. [Result] The results showed that the whole length of the mitochondrial genome sequence of S. pulchella (GenBank accession: MZ157403) is 15 216 bp, which encodes 37 genes: 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes (rrnL and rrnS) and an A + T-rich region. The gene content is consistent with the mitochondrial genome structure of other published Lepidoptera insects. The A+T content of protein-coding genes, whose length is 10 830 bp, is 77.7% . The A+T content of tRNA is 80.9%. The secondary structure of 22 tRNAs is typical of the clover type except trnS1, whose dihydrouracil ring and arm is missing. Phylogenetic analysis of 20 species including two outgroup taxa, was conducted. Bayesian phylogenetic tree shows that the phylogenetic relationships of ygaenidae is: (Zeuzera multistrigata + Endoxyla cinereus)+(((Apoda limacodes + Narosa nigrisigna) + ((Iragoides fasciata + (Thosea sinensis + Thosea sythoffi)) + (Parasa consocia + (Mahanta tanyae+Monema flavescens))))+((Phauda flammans + Zygaena filipendulae + ((Illiberis pruni + Illiberis ulmivora)+(Pidorus atratus + ((Amesia sanguiflua + Rhodopsona rubiginosa)+ Eterusia aedea + (Histia rhodope+Sorita pulchella))))))). The result of Maximum likelihood of phylogenetic tree is: (Endoxyla cinereus + Zeuzera multistrigata + ((Apoda limacodes + Narosa nigrisigna) + ((Iragoides fasciata + (Thosea sinensis + Thosea sythoffi)) + (Parasa consocia + (Mahanta tanyae + Monema flavescens))) + (Zygaena filipendulae + (Phauda flammans + ((Illiberis pruni + Illiberis ulmivora)+(Pidorus atratus + ((Amesia sanguiflua + Rhodopsona rubiginosa)+(Histia rhodope + (Eterusia aedea + Sorita pulchella))))))))). [Conclusion] Zygaenidae and Limacodidae are both monophyletic. The results of phylogenetic analyses showed that Zygaena filipendulae and Phauda flammans differentiate earlier. Illiberis pruni is the sister group of Illiberis ulmivora. Pidorus atratus is monophyletic. Amesia sanguiflua is sister group to Rhodopsona rubiginosa. S. pulchella is sister group to Histia rhodope, and together they are sister group to Eterusia aedea.
Authors and Affiliations
Zhuo ZHAO, Chenyang LIU, Qingfen WENG, Baohai WANG, Qing ZHAI
Keywords
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- EP ID EP769189
- DOI 10.16445/j.cnki.1000-2340.20221110.001
- Views 3
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