Characterization of β-Tubulin cDNA(s) from Catharanthus roseus
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2015, Vol 6, Issue 1
Abstract
Vinblastine and vincristine are anti-tumor drugs produced by different species of Vinca. Vinca alkaloids depolymerize microtubules and as a result interfere with the normal formation of microtubules during cell division. Extremely low amount of vinca alkaloids in Vinca species and increased demand for chemotherapy are motives to scale up its production by biotechnological approaches. Here, β-tubulin gene family in Catharanthus roseus (vinca rosea) was analyzed to provide enough information for further approaches. 24 β-tubulin coding sequences from plants, mainly Arabidopsis thaliana, were obtained from genebank and aligned to design gene specific primers. Total RNA from leaves of C. roseus were isolated, the cDNA(s) synthesized using specific reverse primers, the amplified fragments cloned in pJET cloning vector and sequenced. Eight clones containing partial β-tubulin cDNAs’ of C. roseus; including an almost full-length coding sequence, were constructed. The comparison of sequencing results with β-tubulin mRNA from Arabidopsis thaliana, Arabidopsis lyrata, Populus trichocarpa, Zea maize, Oryza sativa, Hordeum vulgare, Gossypium hirsutum, Nicotiana attenuate, Solanum tuberosum and lycopersicone sculentum revealed about 85% similarity of nucleotide sequence and about 98% identities at amino acid level. The clustalW alignment analyses underlined a short peptide at position 359-367 with substitutions very specific to C. roseus. Based on our sequencing results, we predicted at least four members of β-tubulin gene family for C. roseus. TUB cDNAs’ from C. roseus sequenced to reveal any differentiation in comparison with non-vinca alkaloid producer plants. It was proposed the sites would carry higher potential for any amino acid alteration providing self-protection in vinca alkaloids producer plants; e.g. C. roseus.
Authors and Affiliations
Ziba Fooladvand, Shamsozoha Abolmaali, Abbas Saidi, Hamed Ashourion
Keywords
Related Articles
- EP ID EP38152
- DOI -
- Views 153
- Downloads 0
Distribution patterns and diversity of leaf litter and soil-dwelling arthropods in a lowland rainforest in southern Cameroon
We investigated the effect of forest disturbance and leaf litter depth on arthropod group composition, abundance and density in a lowland rainforest in Cameroon. Arthropods were collected by hand collection and pitfall t...
Studying the ways of recovering debts from a Bankrupt Merchant
Bankruptcy is a situation in which a merchant has become incapable of paying debts. In Iran, before the enactment of the Commercial Code, the provisions relating to insolvency were applied to both merchants and non-mer...
Management consequences and diseases transmission potentials of dredge sediments from Nworie River, Owerri, Eastern Nigeria
Improper disposal of Nworie River dredge sediments can serve as source of pathogenic organisms to the inhabitants of Owerri, eastern Nigeria. This study aimed at determining the bacterial quality of the sediments to he...
Revitalization on local wisdom of Wetu Teli community in forest management of Bayan, Nrth Lombok, West Nusa Tenggara
Participation of Wetu Telu Community in indigenous forests management of Bayan, proved success maintains the forests. It implied in the success of Bayan Area as: 1) the largest number of indigenous forests; 2) the large...
Population dynamics of Rotifera in Ismailia Canal, Egypt
Ismailia Canal is one of the most important tributaries of the River Nile in Egypt. The population dynamics of rotifers in three zones of Ismailia Canal were studied in spring and autumn 2011, in relation to some physic...