CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF THE LEAF ESSENTIAL OIL OF ERYTHROPHLEUM SUAVEOLENS GUILL. AND PERR. (BRENAN) (FAMILY: FABACEAE/ CAESALPINIOIDEAE)
Journal Title: INTERNATIONAL JOURNAL OF MODERN PHARMACEUTICAL RESEARCH - Year 2018, Vol 2, Issue 4
Abstract
The leaf of Erythrophleum suaveolens (E. suaveolens) is applied to wounds and also used to repel pest and insects. However, there is no report on the chemical composition of the leaf essential oil of the plant. This study aims to determine the chemical composition and antimicrobial activity of the essential oil of the leaf of E. suaveolens. The essential oil was obtained by hydrodistillation method with the aid of a Clavenger type apparatus. The GC-MS analysis of the essential oil was done using Shimadzu QP- 2010plus GC-MS. Individual constituents were identified by comparing their mass spectra with known compounds and NIST Mass Spectral Library. The antibacterial activities of the essential oil against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli, Candida albicans and Mycobacterium bovis were evaluated using microdilution broth method using 96-well microplates. Ciprofloxacin and Fluconazole were used as the standard drugs. The minimum inhibitory concentration (MIC) value of the essential oil was also determined. The GCMS analysis showed that the oil contained 10 compounds with α-citral, β-citral and squalene as the major compounds at 36.96%, 25.41% and 20.07% respectively. Other constituents were β-linalool (4.06 %), α-thujenal (3.73 %), hydroxylneoisolongifolane (2.91%), β-myrcene (1.85%), 2-hydroperoxyhexane (1.82%), α-farnesene (1.61%) and 1,2-epoxy-3-propoxy propane (1.59%). The antimicrobial assay showed that the essential oil possessed antimicrobial activity against E. coli with an MIC of 25μg/ml. The isomeric composition of citral in E. Suaveolens is 59.3% α-citral and 40.7% β- citral. The antimicrobial activity of the oil against E. coli may be due to high content of citral.
Authors and Affiliations
Samuel Ehiabhi Okhale
Keywords
Related Articles
- EP ID EP666556
- DOI -
- Views 178
- Downloads 0
DRUG UTILIZATION PATTERN AND POTENTIAL TERATOGENESITY RISK AMONG PREGNANT WOMEN; NEKEMTE REFERRAL HOSPITAL, EAST WOLLEGA ZONE, OROMIA REGIONAL STATE, ETHIOPIA
Back ground: Pregnancy is a special physiological condition where the treatment needs a special concern. It is important to treat the mother whenever needed while protecting the unborn from any side effects to the greate...
AWARENESS ABOUT PSORIASIS DISEASE
A large population is affected by psoriasis disease. A type of itchy skin disease many factors are responsible for the prevalence of the disease, we have conducted a survey on psoriasis disease among the MPHILL students,...
WISDOM TOOTH - ERUPTION: FORENSIC IMPLICATIONS
Wisdom teeth, also called adult’s third molars, are the four permanent third molar teeth, the most posterior of the three molars per quadrant. They are called so as they erupt during the adolescence, between 14 and 21 ye...
SIGNIFICANCE OF ANIMALS IN YOGA POSES
Cat/Cow poses are common poses use together to warm up and stretch. In fact, in India cow is a symbol of love. Krishna who was a cowherd and he loved milk and ghee that essence of motherly love. Performing cat pose regul...
AWARENESS ABOUT HYPERTENSION IN BIOLOGY STUDENTS
Hypertension or high blood pressure is a harmful disease which can increase the pressure of the blood in the arteries to the abnormal level. It also causes damages to the other body parts e. g brain, kidneys, eyes and pa...