CHEMICAL COMPOSITION, ANTIOXIDANT, ANTIBACTERIAL AND CYTOTOXICITY ANALYSIS OF BLUMEA LACERA (BURM. F.) DC
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 8
Abstract
Objective: To investigate the in vitro antibacterial and antioxidant potential of B. lacera (leaves). The most active extracts were examined for their chemical composition and cytotoxicity.Methods: The antibacterial activity of five different extracts were examined against 8 bacterial strains (5 Gram-positive and 3 Gram-negative) using resazurin based microtitre dilution assay (RMDA) and disk diffusion assay (DDA). The antioxidant activity of extracts was demonstrated by using DPPH (1, 1-diphenyl-2-picrylhydrazyl) assay and superoxide radical scavenging assay. Chemical composition and cytotoxicity were assessed by using gas chromatography-mass spectrometry (GC-MS) and haemolytic assay, respectively.Results: According to RMDA, the petroleum ether extract (PEE) and chloroform extract (CE) exhibited highest antibacterial activity. The PEE showed highest activity against Salmonella enterica ser. typhi and Serratia marcescens with MIC i.e. 390.62 μg/ml. Similarly, the CE showed highest antibacterial activity against Bacillus cereus and Micrococcus luteus with MIC i.e. 390.62 μg/ml. In DPPH assay, CE showed the highest radical scavenging activity with IC50 57.46 µg/ml. In GCMS analysis, the principal compounds in PEE and CE were stigmasterol (12.86 %) and L-(+)-ascorbic acid 2, 6-dihexadecanoate (11.73 %), respectively. In haemolytic assay, the PEE and CE showed non-toxic behaviour up to 125 µg/ml and 500 µg/ml, respectively. Conclusion: The present investigation represents B. lacera as an incredible herb. The PEE and CE were found to possess promising antibacterial and antioxidant properties. The CE exhibited lesser toxicity as compared with PEE.
Authors and Affiliations
Savita Khatri, Neetu Phougat, Renu Chaudhary, Bharat Singh, Anil Kumar Chhillar
Keywords
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