Chemical constituents, antioxidant and antibacterial activities of the hexane extract of Alchemilla sericata Reichenb
Journal Title: Trends in Phytochemical Research - Year 2017, Vol 1, Issue 1
Abstract
The hexane extract from the aerial parts of Alchemilla sericata Reichenb. which was collected from Khalkhal-Asalem road, Ardabil Province, northwestern Iran, was obtained using a commercially available Soxhlet apparatus. The fatty acids were derived to methyl esters and determined through gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) systems. The extract was characterized by high amounts of saturated fatty acids (SFA) (66.0%) along with lower quantities of some other terpenoid compounds. Accordingly, the main components of the hexane extract were found to be hexadecanoic acid (41.4%), 9,12-octadecadienoic acid (21.0%), octadecanoic acid (18.0%) and dioctylphthalate (7.7%), respectively. The hexane extract from A. sericata was also detected as a rich source of an important source of palmitic acid compound. In addition, its antioxidant activity was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The results indicate that extracts from the aerial parts of A. sericata possess considerable antioxidant activity. The highest radical scavenging activity was detected in this plant oil (IC50=185 μg/mL). The antimicrobial activity of the hexane extract sample were determined against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae), as well as three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The bioassays used showed that the hexane extract exhibited a moderate antimicrobial activity. This study revealed that the extracts from the aerial parts of this plant are potential sources of fatty acid components, as well as effective natural antioxidants.
Authors and Affiliations
Ali Shafaghat; Akram Panahi, Masoud Shafaghatlonbar
Keywords
Related Articles
- EP ID EP680520
- DOI -
- Views 232
- Downloads 0
Chemical composition and yield of essential oil from two Iranian species of basil (Ocimum ciliatum and Ocimum basilicum)
Basil belonging to the Lamiaceae family, is one of the most important aromatic and medicinal plants, and is widely cultivated in many countries. Areal parts, especially leaves of sweet basil are widely used to enhance th...
Ficusanol, a new cinnamic acid derivative and other constituents from the roots of Ficus exasperata Vahl. (Moraceae) with antioxidant and cytotoxic activities
Phytochemical investigation of the roots of Ficus exasperata Vahl. (Moraceae) led to the isolation of a new cinnamic acid derivative, named ficusanol (1) along with eleven known compounds: 3-(6-methoxybenzo[b]furan-5-yl)...
Chemical profiling of the fruits of Styrax officinalis L. from Monti Lucretili (Latium region, Central Italy): Chemotaxonomy and nutraceutical potential
In this work, the first phytochemical analysis ever performed on the fruits of Styrax officinalis L. (Styracaceae) collected from a population vegetating in the Mounts Lucretili National Park (Italy) was reported. Fiftee...
Evaluation of cholesterol lowering property of selected herbs in ghee (heat clarified milk fat)
Ghee is a heat clarified milk fat and supply various nutrients. It contains cholesterol which is considered to be associated with various diseases. To the best of our knowledge, the present work focusing on reduction of...
Nano-encapsulation of thyme essential oil in chitosan-Arabic gum system: evaluation of its antioxidant and antimicrobial properties
In this study Nano–capsules based on chitosan (CS) and Arabic Gum (GA) with Thyme essential oil (TEO) as an active ingredient were prepared using emulsion method. The nano–capsules were characterized by their Encapsulati...