Chemical Mutagenesis of Bacillus subtilis for Improved Mannanase Biosynthesis
Journal Title: Journal of Advances in Microbiology - Year 2017, Vol 3, Issue 2
Abstract
The aim of the present study was to isolate bacterial associated with abattoir waste water, compost saw dust, soil and water samples from Ilaje Lake, Ondo State, Nigeria. The microbial isolates were identified using standard microbiological method. The bacterial isolates were screened for mannanase production. Mannanase activity was determined by dinitrosalicylic acid (DNSA) method while protein concentration in the fermentation broth was quantified by Lowry’s method. Isolate designated 2k tentatively identified as Bacillus subtilis had the highest mannanase activity. The isolate with highest mannolytic activity was then subjected to different mutagens. The mutants of Bacillus subtilis generated were screened for mannanase production in comparison with the wild type in submerged state fermentation. All the mutant strains generated from B. subtilis had their mannolytic activities repressed in comparison with the wild strain. Out of mutants screened, mutant designated CH017 have the highest mannolytic activity 1.20 U/mg. The mannanase activity produced by CH017 was approximately 44% lower than the wild strain. The pretreatment of B. subtilis with nitrous acid caused enzyme repression. Therefore, another chemical mutagen should be worked upon whether it would result in appreciable yield of mannanase.
Authors and Affiliations
B. S. Adeleke, S. O. Ojo, Y. D. Oluwafemi, O. O. Olaniyi
Keywords
Related Articles
- EP ID EP338142
- DOI 10.9734/JAMB/2017/30911
- Views 93
- Downloads 0
Antibiotic Susceptibility Profile of Bacterial Pathogens Isolated from Malabor Hostel Tap Water, Calabar- Nigeria
The antibiotic resistance and susceptibility profiles of some bacterial isolates including Listeria monocytogenes, Erwinia stewartii, Legionella pneumophilia, Carnobacterium gallinarum, Staphylococcus caseolyticus, Enter...
Antibiotic Susceptibility and Plasmid Profile of Escherichia coli from Door Handles in Two Tertiary Institutions in Nasarawa State, Nigeria
Aim: This study is aimed at isolation, antibiotic susceptibility and plasmid study of Escherichia coli isolates from door handles in the study location. Study Design: Cross-sectional study. Place and Duration of Study: T...
Aspergillus Xylanases
There has been tremendous growth and development in the use of xylanase in the biotechnology industry. The market trends reveal that xylanase takes major position of share up to 20% of the world enzymes market along with...
Baculoviruses: Emerging Frontiers for Viral Biocontrol of Insect Pests of Agricultural Importance
Accumulated evidences gathered over recent decades demonstrated that Baculoviruses (Occlusion body forming nucleopolyhedroviruses and the host specific granuloviruses) have proven Biocontrol activities on insect and pest...
Molecular Characterization of ESBLs Genes among Multi-drug Resistant Klebsiella species in Ile-Ife South-West, Nigeria
Aim: This study was carried out to determine the presence of ESBLs genes (TEM, CTX-M, SHV, AAC and OXA types of Beta-lactamases) in clinical isolates of Klebsiella species producing extended spectrum beta-lactamase. Ext...