Chemical Structural Alterations of Root Surface After Er: YAG Laser, Nd: YAG Laser Irradiation-An Infrared Spectroscopic Study
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 3
Abstract
AIM: To assess the chemical structural alterations of root surface by using Fourier Transform Infrared Spectroscopy followed by Er: YAG and Nd: YAG laser irradiation. MATERIAL AND METHOD: Fifty five upper incisor teeth extracted from patients attending the outpatient Department of Periodontics, Tamilnadu Govt. Dental College and Hospital Chennai -3. 110 specimens of size 3mm x 4mm x 1mm were prepared. For evaluation fifty five samples were divided into three groups. Group A,B and C. Group A contain five samples which serve as non irradiated control. Group B and Group C further divided into five sub groups and each subgroup contains five samples. All the specimens within the subgroups of B and C irradiated with 100 mJ, 200 mJ, 300 mJ, 400 mJ, 500 mJ of Er:YAG laser and 211.66 J/cm 2 , 423.33 J/cm 2 , 635 J/cm 2 , 846.66 J/cm 2 1058.33 J/cm 2 of Nd:YAG laser respectively. For chemical analysis (FTIR spectroscopy) of root remaining fifty five samples were divided into Group D (non irradiated control) , group E and F. All the sub groups of Group E and F were irradiated with same parameters used in Group B and Group C respectively. Chemical structural changes after laser irradiation were performed on Fourier Transform Infrared Spectroscopy instrument. The data obtained was statistically analyzed by one-way ANOVA multiple range test by Turkey- HSD and Mann Whitney tests. RESULTS: Er: YAG laser at 100 mJ effectively remove smear layer without crater formation.. The Nd:YAG laser at the energy density of 211.66 J/cm2 and 423.33J/cm2 remove the smear layer at the energy density of 1058.33 J/cm 2 showed visible charring and FTIR spectroscopy profile showed reduction in peak height of major bands (Amide I, II III, OH group and phosphate) after 300 mJ of Er:YAG laser. The reduction in peak intensity observed only beyond the 846.66 J/cm2 of Nd:YAG laser irradiation and new absorption band was noticed (2010 cm-1 and 2017 cm-1 ) at samples treated with 846.66 J/cm2 and 1058.33 J/cm 2 of Nd: YAG laser. CONCLUSION: Er:YAG at lower energy density effectively remove smear layer without production of toxic substance when compared to Nd:YAG laser.
Authors and Affiliations
Dr. R. Karthikeyan, Dr. A. J. Anand, Dr. Pradeep kumar yadalam, Dr. P. R. Ganesh, Dr. A. Muthukumarasamy, Dr. K. Malathi
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