CHLORAMBUCIL -TREATMENT INDUCED OXIDATIVE STRESS IN TESTICULAR TISSUE OF SWISS ALBINO MICE
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2019, Vol 6, Issue 6
Abstract
Chlorambucil (CLB) is an alkylating agent used against chronic leukemia. This study was carried out to investigate the adverse effect of chlorambucil treatment on testicular tissue of mice. Twenty Swiss albino mice were randomized divided into four groups (n=5). Group 1 served as control received vehicle while 2, 3 and 4 groups received 1mg/kg, 3mg/kg and 5mg/kg body weight of chlorambucil (CLB) respectively for 28 days via intraperitoneal injection. Testosterone and Luteinizing hormone were estimated. Oxidative and anti-oxidative biomarker enzymes such as lipid peroxidation (LPO), catalase (CAT), superoxide dismutase (SOD) and reduced glutathione (GSH) levels were evaluated. Effect on sperm parameters was carried out. The results showed that treatment with chlorambucil at 3mg/kg and 5mg/kg caused a significant increase at p>0.05 in the luteinizing hormone and testosterone levels. CLB at 3mg/kg and 5mg/kg body weight caused significant increase in LPO with simultaneous decrease in CAT, SOD and GSH levels. The findings revealed that chlorambucil treatment resulted in generation of oxidative stress in the testicular tissue of treated mice in dose dependent manner.
Authors and Affiliations
Olajumoke Omolara Ojo
Keywords
Related Articles
- EP ID EP670899
- DOI -
- Views 162
- Downloads 0
USE OF GENOTYPE MTBDR ASSAY FOR MOLECULAR DETECTION OF RIFAMPIN AND ISONIAZID RESISTANCE IN MYCOBACTERIUM TUBERCULOSISCLINICAL STRAINS ISOLATED IN SUDAN
Introduction: The most effective first line drug against Mycobacterium tuberculosis are rifampicin and isoniazid, mutations in several gene determinant resistance Mycobacterium tuberculosis to isoniazid and most common g...
BALANCING THE ACT-SELF MEDICATION WITH ANTIBIOTICS
Self-medication is defined as the use of drugs for the treatment of self-diagnosed disorders or symptoms or the continued use of prescribed drug for chronic or recurrent disease or symptoms. In developing countries like...
A REVIEW: SUSTAINED RELEASE ORODISPERSIBLE TABLET
In the 1980s, orally disintegrating tablets (ODTs) were introduced in the market becoming one of the most useful techniques of drug delivery. The target populations for ODTs are bedridden pediatric, geriatric, and or dis...
COMPARISON OF EFFICACY AND SAFETY OF TWO ARTIFICIAL TEARS IN PATIENTS WITH MILD TO MODERATE DRY EYE SYMPTOMS: A RANDOMIZED, PARALLEL STUDY
Purpose: Artificial tears are commonly used for symptomatic treatment of dry eye disease (DED). We compared the efficacy and safety of two preservative-free lubricant eye drops; Osmodrops (carboxymethylcellulose 0.5%, gl...
INVESTIGATION OF THE ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF PHYLLANTHUS ACIDUS IN COMBINATION WITH DIFFERENT CLASSES OF ANTIBIOTICS AGAINST SINGLE AND MULTIDRUG RESISTANCE STRAINS
The aim of this project work was to evaluate the Antimicrobial Activities of phyllanthus acidus (Family: Phyllanthaceae). Antimicrobial activity of chloroform and ethyl acetate extract leaves of phyllanthus acidus was te...