Cholera toxin A1 residues single alanine substitutional mutation and effect on activity with stimulatory G protein
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 6
Abstract
Cholera is a well-known gastrointestinal infection. The cholera toxin is an important pathological substance in pathogenesis of cholera diarrhea. Cholera toxin is composed of catalytic A1 subunit, an A2 linker, and a homopentameric cell-binding B subunit. In enterocyte, cholera toxin will attach to GM1 ganglioside receptors on the apical membrane and causes retrograde vesicular trafficking to endoplasmic reticulum. At endoplasmic reticulum, cholera toxin A1 is released from the rest of the toxin into cytoplasm. The cholera toxin A1 interacts will catalyze ADP ribosylation of subunits of stimulatory G protein resulting a persistent activation of adenylate cyclase and an elevation of intracellular cAMP which further result in diarrhea. The single alanine substitutional mutation can result in the reduction of the interaction activity between cholera toxin A1 and stimulatory G protein. In this study, the four well-known mutations, H55, R67, L71, S78, or D109, of cholera toxin A1 is focused. The author hereby calculates for the reaction energy for the reaction between cholera toxin A1 and stimulatory G protein in na¨ıve case and mutated case. To calculate, the standard bonding energy calculation technique in mutation analysis was used. It can be seen that aberrant in reaction energy in each studied mutation is different and can imply the different effect on activity with stimulatory G protein.
Authors and Affiliations
Somsri Wiwanitkit, Viroj Wiwanitkit
Keywords
Related Articles
- EP ID EP254516
- DOI 10.1016/j.apjtb.2017.05.012
- Views 64
- Downloads 0
Triterpenoid of avocado (Persea americana) seed and its cytotoxic activity toward breast MCF-7 and liver HepG2 cancer cells
Objective: To determine the structure of triterpenoid isolated from avocado seeds and the cytotoxic effect on MCF-7 and HepG2 cells. Methods: The powder sample was macerated with ethanol, followed with separation of the...
Therapeutic effect of soluble worm protein acting as immune regulatory on colitis
Objective: To investigate the anti-inflammatory effect of the protein derived from the soluble factor of Heligmosomoides polygyrus (H. polygyrus) excretory-secretory in a colitis model. Methods: Colitis was induced by pr...
Aqueous extracts of microalgae exhibit antioxidant and anticancer activities
Objective To investigate the antioxidant and anticancer activities of aqueous extracts of nine microalgal species. Methods Variable percentages of major secondary metabolites (total phenolic content, terpenoids and al...
The distribution of HCV in subjects attending hospitals in Duhok City, Iraq
Objective: To investigate the prevalence of hepatitis C virus (HCV) and its genotypes in Duhok City, Kurdistan Region, Iraq. Methods: A cross sectional study was conducted to investigate the prevalence of HCV and its gen...
Disseminated strongyloidiasis in an immunocompromised host: A case report
Infections caused by Strongyloides stercoralis (S. stercoralis) in human are generally asymptomatic, however in immunocompromised individual, hyperinfection may develop with dissemination of larvae to extra-intestinal or...