CHOLESTATIC LIVER FIBROSIS IN A RAT MODEL OF BILE DUCT LIGATION: EVALUATING BIOCHEMICAL VERSUS HISTOPATHOLOGICAL CHANGES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 6
Abstract
Objective: Bile duct ligation (BDL), chronic liver injury model, was extensively used in studying mechanisms of fibrogenesis and antifibrotic agents. Considering the liver regenerative capacity and the diverse results from BDL, the present study aimed to evaluate the biochemical and histopathological changes over 10 weeks following BDL assessing if BDL-induced changes remain in a deterioration state or improve at a certain stage.Methods: Sham operation and BDL were conducted in Male Wistar rats. Serum AST, ALT, total bilirubin and albumin and hepatic hydroxyproline (HYP), reduced glutathione (GSH) and malondialdehyde (MDA) were measured in sham-operated (n=3) and BDL-rats (n=6) at 0, 1, 2, 4, 6, 8 and 10 weeks following operation. Liver tissue was also processed for histopathological analysis (H&E and Sirus red staining).Results: Progressive liver injury (H&E) and collagen deposition (Sirus red and HYP) in BDL-rats were observed starting from the first week post-operation and reached their maximum with early signs of cirrhosis on the 10th week of BDL. Severe and sustained cholestatic injury appeared in 2 weeks (increased ALT, AST, bilirubin along with decreased albumin (P<0.001) compared to sham-operated rats). AST peaked on first week, however, bilirubin, ALT and MDA peaked on the 4th week (P<0.001) then gradually decreased compared to their peaks.Conclusion: The relative improvement in liver function/cholestasis following their peaks in BDL model despite progression of fibrosis and hepatic injury require investigators using this model to consider not only biochemical, but also histopathological findings to guarantee an accurate interpretation of their results.Â
Authors and Affiliations
Hoda E Mohamed, Sahar E Elswefy, Laila A Rashed, Nahla N Younis, Mohamed A Shaheen, Amal M H Ghanim
Keywords
Related Articles
- EP ID EP578717
- DOI -
- Views 64
- Downloads 0
REGULATION OF ETHYL ACETATE FRACTION FROM MORINGA OLEIFERA LEAVES TO IMPROVE LIPID METABOLISM AND INSULIN SENSITIVITY IN TYPE 2 DIABETES
Objective: Moringa oleifera is a medicinal plant species that has potential as an antidiabetic. The present study was designed to evaluated molecullar mechanism antidiabetic ethyl acetate fraction of Moringa oleifera lea...
UV SPECTROPHOTOMETRIC ESTIMATION OF SUNITINIB MALATE IN PHARMACEUTICAL DOSAGE FORM
Objective: To develop and validate simple zero (D0), first (D1) and second (D2) order derivative UV Spectrophotometric methods for the determination of Sunitinib malate in pharmaceutical dosage form.Methods: Sunitinib ma...
ENHANCEMENT OF SOLUBILITY AND DISSOLUTION RATE OF TELMISARTAN BY TELMISARTAN-OXALIC ACID CO-CRYSTAL FORMATION
Objective: Telmisartan (TMS), an angiotensin II receptor antagonist used for the prevention and treatment of hypertension has a very low solubility in water, so its dissolution rate is low. The aim of this study is to im...
ANTI ISCHEMIA REPERFUSION EFFECT OF SODIUM THIOSULFATE IN LLC PK1 CELLS
Objectives: Hydrogen sulphide (H2S) has a protective effect against renal ischemia reperfusion injury (I/R), but it is toxic and have the limitation for its controlled in-vivo release to the system. However, its metaboli...
1,3,4-THIADIAZOLE: A BIOLOGICALLY ACTIVE SCAFFOLD
There has been considerable interest in the development of novel compounds with anticonvulsant, antidepressant, analgesic, anti-inflammatory, anti allergic, antipsychotic, antimicrobial, antimycobecterial, antitumour, an...