Choosing the Best Microalgae and Optimising its Culture Medium to Produce More Starch for Medicinal Use
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 5
Abstract
ABSTRACT Introduction: Some species of microalgae have the ability to produce high amount of carbohydrates, rather than lipids. The extracted starch obtained from the ultrasonic treated green microalgae has high level of antioxidant activities and in animal studies, the extracted starch from Chlorella vulgaris (C. vulgaris) exhibited anti-atherogenic, anti-cholesterolemic, anti-inflammatory and anti-tumour effects. The green microalga has been widely used as a food source containing a complete set of nutrients including carbohydrates, proteins, vitamins, and minerals. Aim: To optimise the microalgae culture medium to increase starch production for medicinal use. Materials and Methods: This study was conducted as a field, observational and experimental research. For this purpose, 5 mL of microalgae C. vulgaris, Scenedesmus dimorphus (S. dimorphus) and Scenedesmus quadricauda (S. quadricauda), in the test tube containing Bold’s Basal Medium (BBM). BBM and Z8 media were used to grow freshwater microalgae. Light absorbance, cell count and biomass of microalgae in these media were studied. The experimental design method based on Taguchi method was conducted for optimising medium for microalgae with highest starch production, with light intensity, nitrogen and phosphorus factors, in three levels (1, 0,-1). Results: Specific growth rates of C. vulgaris in the media BBM and Z8 were 0.075 and 0.055 d-1, and for S. quadricauda and S. dimorphus were 0.072 and 0.008; 0.038 d-1, respectively. Due to significant difference (p=0.034) between Z8 and BBM media, BBM was selected for growing C. vulgaris, S. quadricauda (p=0.308) and S. dimorphus (p=0.033). In addition, growth rate and starch production of desired microalgae were achieved. Starch content in S. dimorphus, C. vulgaris and S. quadricauda were 19%, 29% and 20% of biomass weight, respectively. Conclusion: It was found that starch content was increased with increasing light, reducing phosphorus and nitrogen in Chlorella and the results showed that C. vulgaris could be a good choice as a starter for producing starch for medicinal purpose.
Authors and Affiliations
Hamid Ramezani Awal Riabi, Hamed Ramezani Awal Riabi
Keywords
Related Articles
- EP ID EP522381
- DOI 10.7860/JCDR/2018/29154.11496
- Views 51
- Downloads 0
Gestational Urinary Incontinence in Nulliparous Pregnancy- A Pilot Study
Introduction: Urinary Incontinence (UI) in pregnancy is more than a disease; it is a social problem that necessitates special care and management. The exact rationales and biological facts behind urinary incontinence dur...
Molecular and Phenotypic Identification and Speciation of Malassezia Yeasts Isolated from Egyptian Patients with Pityriasis Versicolor
Introduction: Pityriasis Versicolor (PV) is a common health problem caused by genus Malassezia, a lipophilic fungi found as a part of the normal flora of skin. Although PV is common in Egypt, there is little information...
Purple Urine Bag Syndrome: Series of Nine Cases and Review of Literature
ABSTRACT Purple coloured urine occurs in the patients, mainly elderly who are on urinary catheters for a long-time and develop Purple Urine Bag Syndrome (PUBS), wherein the urinary bag and the tubing turn in to purple co...
Er:YAG and Diode Lasers in Treatment of Peri-Implantitis–A Case Report
ABSTRACT One of the leading causes of dental implant loss in the present time is peri-implantitis. This paper describes a clinical case of peri-implantitis treatment using Er:YAG and a 980-nm diode laser. In the present...
Comparative Evaluation of Propofol Fentanyl, Propofol-Midazolam and Propofol-Dexmedetomidine on Haemodynamic and Postoperative Recovery for Endoscopic Retrograde Cholangiopancreatography
Introduction: Adequate patient sedation is mandatory for Endoscopic Retrograde Cholangiopancreatography (ERCP). Recent studies indicate that propofol and combination of midazolam or fentanyl or dexmedetomidine is superio...