CHROMATOGRAPHIC AND SPECTROPHOTOMETRIC ANALYSIS OF BIOACTIVE COMPOUNDS FROM CAYRATIA TRIFOLIA (L.) STEM
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 6
Abstract
Objective: The present study was to analyze the bioactive compounds from stem ethanolic extract of Cayratia trifolia by FTIR, HPTLC and GC-MS techniques.Methods: The FTIR was applied and infrared spectrum in mid-infrared region 4000-400 cm-1 was used, HPTLC fingerprinting profiles was done by using Hamilton syringe and CAMAG LINOMAT 5 instrument and GC-MS analysis of stem ethanolic extract of Cayratia trifolia (L.) was performed using the equipment Agilent technologies 7890 A.Results: The FTIR analysis identified the functional groups such as amine, acid, alkane, ketone acyclic, carbonyl, aromatic, ester and alkene. HPTLC fingerprinting profile proves the presence of alkaloids, flavonoids, glycosides, saponin and steroids. GC-MS revealed the presence of various compounds like hexadecanoic acid-ethylester, phytol, tetratetracontane, stigmasterol, nonacosane and octadecane-1-bromo-in stem ethanolic extract of Cayratia trifolia.Conclusion: In conclusion, Cayratia trifolia plant stems ethanolic extract holds more bioactive compounds that may lead to the development of novel drug against various diseases and disorders.Keywords: Cayrtaia trifolia, FTIR Spectroscopy, HPTLC analysis, GC-MS technique, Bioactive compound
Authors and Affiliations
Sundaram Sowmya, Palanisamy Chella Perumal, Velliyur Kanniappan Gopalakrishnan
PHYTOCHEMICAL SCREENING AND COMPARISON OF ANTIOXIDANT ACTIVITY OF WATER AND ETHANOL EXTRACT PROPOLIS FROM MALAYSIA
Objective: In an attempt to compare extraction yield, phytochemical screening, radical scavenging activity, and total phenolic and flavonoid contents of water extract propolis (WEP) and ethanol extract propolis (EEP) fro...
THE ANTI–ANGIOGENIC ACTIVITY OF PHOENIX DACTYLIFERA SEEDS EXTRACTS
Objective: To identify the possible anti–angiogenic activity of Phoenix dactylifera seeds extract.Methods: The powder of the date palm seeds was extracted sequentially with petroleum ether, chloroform, methanol and wat...
FORMULATION DESIGN AND IN VITRO EVALUATION OF BILAYER SUSTAINED RELEASE MATRIX TABLETS OF DOXOFYLLINE
Objective: To develop bilayer matrix tablet of Doxofylline by providing a loading dose followed by the maintenance dose that suppose to enhance the therapeutic efficacy the drug for acute and sustainable asthma.Methods:...
GC-MS PROFILE OF IN VIVO AND IN VITRO SHOOTS OF CLEOME GYNANDRA L.
Objective: Investigation of the bioactive compounds from the ethanol shoot extracts of in vivo and in vitro plants of Cleome gynandra (C. gynandra) through GC-MS analysis. Methods: The nodal explants were cultured on Mur...
ANTI-INFLAMMATORY, ANALGESIC AND ANTIOXIDANT ACTIVITIES OF HYDRO-ACETONIC EXTRACT FROM WISSADULA AMPLISSIMA VAR. ROSTRATA (SHUM. AND THONN.) R. E. FRIES
Objective: The present study aims to investigate anti-inflammatory, analgesic and antioxidant effects of the hydroacetonic extract (HAE) from Wissadula amplissima whole plant and to determine its polyphenol and flavonoid...