Cisplatin. Determination in workplace air with high performance liquid chromatography
Journal Title: Podstawy i Metody Oceny Środowiska Pracy - Year 2016, Vol 32, Issue 1
Abstract
Cytostatic drugs are a specifi c group of chemotherapy drugs whose toxicity for patientsundergoing treatment is reasonably well understood. However, there is no data on theharmful effects of the impacts of these chemicals on exposed persons during preparation andapplication of anticancer drugs.The aim of this study was to develop a method for determining an inhalable aerosol fraction ofcisplatin in workplace air at the level of 0.2 mg/m3, which is 1/10 of allowable concentration level of2 ug/m3 proposed by the ACGIH.This method is based on sampling of inhalable fraction of cisplatin from the air on the fi lterglass fi ber. After extracting with distilled water, derivative with sodium diethyldithiocarbamate(NaDDTC) was analyzed with high performance liquid chromatography with spectrophotometricdetection. Analysis was performed on reverse phase (mobile phase – methanol: water) usingan analytical column with modifi ed silica gel column (18).On the basis of performed studies, measuring range of 0.03–10 mg/ml was determined whichgave 0.18–62.5 ug /m3 when downloading 960 L of air and 0.25–83.3 ug/m3 when downloading720 L of air. The resulting calibration curve was linear as demonstrated by the regressioncoeffi cient of 0.996. The overall precision of the study was 6.25% and expanded uncertainty of themethod was 27.71.The results and validation parameters confi rm the usefulness of newly developed method fordetermining cisplatin to determine concentrations of this substance in workplace air to evaluateoccupational exposure in a wide concentration range of 0.03–10 ug/ml. The developed methodof determining cisplatin in workplace air has been recorded as an analytical procedure for measuring range of 0.03–0.5 mg/ml which corresponds to 0.1–1.6 NDS value when downloading the 960 L of air and from 0.125–2 when downloading 720 L of air.
Authors and Affiliations
MAŁGORZATA SZEWCZYŃSKA, Małgorzata Pośniak, Sylwia Krzemińska
Keywords
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