Clinical Methicillin-Resistant Staphylococcus aureus May Transfer from Hospital to the Community Through Foods
Journal Title: Vajira Medical Journal: Journal of Urban Medicine - Year 2022, Vol 66, Issue 6
Abstract
Objective: To investigate the capability of methicillin-resistant Staphylococcus aureus (MRSA) and mecA-carrying Staphylococcus aureus (MCSA) transfer from hospital to community through foods due to the mecA gene is responsible for various antimicrobials resistance. Methods: We investigated four MRSA from patients and healthy carriers and one mecA-carrying S. aureus (MCSA) from food, whether they were capable of surviving through acidic condition and simulated gastrointestinal system. All bacterial strains were examined in green papaya salad’s liquid portion (GPL), pH 2.0 and pH 3.0 to test their toleration ability in acidic food. Bacterial toleration to gastrointestinal system was investigated using 0.3% (w/v) pepsin-supplemented phosphate buffer saline (PBS) (pH 2.0 and pH 3.0), and different concentrations bile salt-supplemented tryptic soy broth (TSB). T-test was used to compare the bacterial survival rates at room temperature and 4ºC, before exposure to GPL, pH 2.0 and pH 3.0. Results: The results revealed that MRSA and MCSA could tolerate in GPL, pH 3.0 and pH 2.0 for 2 h and 1 h, respectively. Bacterial exposure to 4ºC for 3 h before incubated in GPL, pH 2.0, significantly prolonged bacterial survival (P < 0.05). Toleration to simulated gastrointestinal system demonstrated that clinical MRSA strain PSU20 well tolerated to simulated gastric juice, pH 3.0 [0.3% (w/v) pepsin] for at least 1 h with the bacterial survival populations of 4.40 log CFU/ml. In addition, this PSU20 well tolerated to all concentrations of bile salts. Conclusion: This study suggests that clinical MRSA has potential to transfer from hospital to community through foods and is able to break the gastrointestinal innate immunity establishing infection in human. This is crucial for public health stand point.
Authors and Affiliations
Pharanai Sukhumungoon Sunisa Dangsriwan Pattamarat Rattanachuay
Keywords
Related Articles
- EP ID EP723934
- DOI http://dx.doi.org/10.14456/vmj.2022.42
- Views 63
- Downloads 0
A Comparative Study between Modified Kakita Pancreaticojejunostomy and Conventional Reconstruction after Pancreaticoduodenectomy: A Retrospective Review
Background: Advances in surgical techniques and appropriate perioperative management has decreased the mortality rate of pancreaticoduodenectomy less than 5 percent. However, postoperative pancreatic fistula is the most...
Exploring Factors Associated with Intolerance of Helmet Noninvasive Ventilation in High-Risk Postextubation Patients
OBJECTIVE: Studies on the use of helmet noninvasive ventilation (NIV) to prevent postextubation respiratory failure in high-risk patients are limited compared with other types of NIV. Only one randomized controlled trial...
Hope of Parents in Children with Hearing Impairments: A Qualitative Study
Background: Hope are related to individual perception and ways to reach their goals. Parents who have high level of hopes can cope with their child disability better than parents who are hopeless. Objective: To explore...
Parent-Adolescent Relationship Patterns of Junior High School in Ubonratchathani Province and Its Association with Family Factors
Objective: To study parent-adolescent relationship patterns of junior high school students in Ubonratchathani Province and its association with family factors. Methods: A cross-sectional descriptive study was conducted...
Association between Hand Grip Strength and Risk of Malnutrition in Elderly at Out-Patient Clinic, Faculty of Medicine, Vajira Hospital
Objectives: To study the optimal cut-off point of hand grip strength for evaluation risk of malnutrition, and factors associated with malnutrition in elderly out-patient clinic, Faculty of Medicine Vajira Hospital. Meth...