Clonal micropropagation of peppermint (Mentha piperita L.) varieties of Ukrainian breeding
Journal Title: Plant varieties studying and protection - Year 2016, Vol 0, Issue 2
Abstract
Purpose. Developing technology for clonal micropropagation of peppermint (Mentha piperita L.) plants of Ukrainian breeding based on the complex of methods of isolated tissue and organ culture in vitro. Methods. During the experiment, such methods as isolated tissue and organ culture in vitro, clonal micropropagation, detached scion grafting, chemotherapy with adding of virucide Ribavirin to the nutrient medium, biometric and statistical ones were used. Results. The stepped procedure of sterilization that we have developed allows to receive 88–100% of sterile explants. For M. piperita L. introduction into culture and clonal micropropagation, Murashige and Skoog (MS) nutrient medium appeared to be optimal supplemented with 6-benzylaminopurine (0.75 mg/l), adenine (0.05 mg/l), indole-3-acetic acid (IAA) (0.05 mg/l) and gibberellic acid (0.5 mg/l) on which the reproduction ratio on the 28th day ranged between 1:7 and 1:15. For recovery of plants from viral infection, virucide Ribavirin at concentration of 10 mg/l was added to the nutrient medium. The proposed nutrient medium for rhizogenesis, that contained IAA (0.5 mg/l) and indole butyric acid (IBA) (0.5 mg/l), allows to obtain the frequency of rhizogenesis up to 84–100%. Regenerated plants were adapted to the conditions in vivo on substrate peat : universal soil : perlite : sand in the ratio 2:1:1:1. The survival rate for peppermint varieties amounted to 96–100%. Conclusions. Biotechnological scheme was developed that permits to get healthy, purebred planting material and intensively propagate plants for supplying breeding programs of the Experimental Station for Medicinal Plants of the Institute of Agroecology and Environmental Management of the National Academy of Agrarian Sciences of Ukraine, among which such varieties as ‘Lebedyna pisnia’ and ‘Ukrainska pertseva’ were selected as the most promising for clonal micropropagation.
Authors and Affiliations
T. Ye. Talankova-Sereda, Yu. V. Kolomiets, I. P. Hrygoriuk
Keywords
Related Articles
- EP ID EP260100
- DOI 10.21498/2518-1017.2(31).2016.70277
- Views 112
- Downloads 0
Productivity of seed agrocenosis of common millet (Panicum miliaceum L.) at varying so ing w terms and techniques under the conditions of Right- Bank Forest-Steppe
The objective of the research is to improve the technology of growing high-quality seeds of millet broomcorn by means of the optimization of sowing terms and methods, aimed at increasing its productivity and improving s...
Molecular selection of sugar beet (Beta vulgaris L.) by resistance to biotic and abiotic factors
Molecular selection (MAS) makes the newest trend in breeding that has been progressively applied in many agricultural crops, including sugar beet. The article reviews papers on sugar beet MAL in Russia and overseas consi...
Consumptive qualities of different potato varieties
Purpose. To summarize results of studying consumptive qualities of different varieties of potato and define basic characteristics which allow to subsume them under specific economic categories. Methods. Field study, lab...
On streamlining the Ukrainian names of plants. Information 6. Names of some subtribe Malinae Reveal taxa (information 6)
Purpose. To analyse the Ukrainian nomenclature of subtribe Malinae representatives and improve terminological system of the Ukrainian names of a species and a generic levels and names of fruit crops. Results. There is a...
Bioecological peculiarities of mid-ripening varieties of strawberry (Fragaria ananassa Duch.) in the northern part of the Forest-Steppe zone of Ukraine
Purpose. Improvement of a range of strawberry varieties based on comprehensive study and introduction of new Ukrainian and West European cultivars. Methods. Cultivars and elite forms were studied using the methods of pr...