Clonal micropropagation of peppermint (Mentha piperita L.) varieties of Ukrainian breeding
Journal Title: Plant varieties studying and protection - Year 2016, Vol 0, Issue 2
Abstract
Purpose. Developing technology for clonal micropropagation of peppermint (Mentha piperita L.) plants of Ukrainian breeding based on the complex of methods of isolated tissue and organ culture in vitro. Methods. During the experiment, such methods as isolated tissue and organ culture in vitro, clonal micropropagation, detached scion grafting, chemotherapy with adding of virucide Ribavirin to the nutrient medium, biometric and statistical ones were used. Results. The stepped procedure of sterilization that we have developed allows to receive 88–100% of sterile explants. For M. piperita L. introduction into culture and clonal micropropagation, Murashige and Skoog (MS) nutrient medium appeared to be optimal supplemented with 6-benzylaminopurine (0.75 mg/l), adenine (0.05 mg/l), indole-3-acetic acid (IAA) (0.05 mg/l) and gibberellic acid (0.5 mg/l) on which the reproduction ratio on the 28th day ranged between 1:7 and 1:15. For recovery of plants from viral infection, virucide Ribavirin at concentration of 10 mg/l was added to the nutrient medium. The proposed nutrient medium for rhizogenesis, that contained IAA (0.5 mg/l) and indole butyric acid (IBA) (0.5 mg/l), allows to obtain the frequency of rhizogenesis up to 84–100%. Regenerated plants were adapted to the conditions in vivo on substrate peat : universal soil : perlite : sand in the ratio 2:1:1:1. The survival rate for peppermint varieties amounted to 96–100%. Conclusions. Biotechnological scheme was developed that permits to get healthy, purebred planting material and intensively propagate plants for supplying breeding programs of the Experimental Station for Medicinal Plants of the Institute of Agroecology and Environmental Management of the National Academy of Agrarian Sciences of Ukraine, among which such varieties as ‘Lebedyna pisnia’ and ‘Ukrainska pertseva’ were selected as the most promising for clonal micropropagation.
Authors and Affiliations
T. Ye. Talankova-Sereda, Yu. V. Kolomiets, I. P. Hrygoriuk
Keywords
Related Articles
- EP ID EP260100
- DOI 10.21498/2518-1017.2(31).2016.70277
- Views 117
- Downloads 0
Foliar feeding of rootstocks of various cultivars of hazelnuts (Corylus maxima Mill.) in the mother plantation for horizontal vegetative propagation
Purpose. Determination of optimum dozes of foliar feeding for each cultivar that will provide the highest output of standard rootstocks. Methods. Field, analytical and statistical ones. Results. The author presents the...
Considering issue of regulating of Ukrainian names of plants. Communication 1. Names for Vaccinium species
Nomenclature of genus Vaccinium considered, as well as the application of names for indigenous and introduced species and denomination of fruit crops as derived from those species. System of binary species names for Vacc...
Seed quality and productive characteristics of O-type sugar beet lines under low temperatures
500 O-type lines of sugar beet hybrid components were studied according to their main economically valuable characteristics, namely, one-seed structure, germination readiness, productivity, sugar content and sugar yield....
Bioecological peculiarities of mid-ripening varieties of strawberry (Fragaria ananassa Duch.) in the northern part of the Forest-Steppe zone of Ukraine
Purpose. Improvement of a range of strawberry varieties based on comprehensive study and introduction of new Ukrainian and West European cultivars. Methods. Cultivars and elite forms were studied using the methods of pr...
Breeding durum winter wheat for adaptive properties improvement
The results of breeding (1995–2013) multiline varieties of durum winter wheat are presented: their advantages as compared with standards for winter hardiness and drought resistance, yield, disease resistance, macaroni pr...