Cloning and gene expression of cytochrome P450 gene from Alcanivorax borkumensis Bacterium
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2014, Vol 2, Issue 1
Abstract
Alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. The ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. A. borkumensis have two genetic systems for alkane biodegradation. The First system is alkane hydroxylase (alk-B1 and alk-B2) and the second system is cytochrome P450. Until now there is not any report on cloning of cytochrome P450 gene of A. borkumensis. In this study cytochrome P450 gene from A. borkumensis was cloned. At first P450 gene was cloned in pBluescript plasmid by blunt cloning and insertion was confirmed by colony PCR. Then P450 gene was cloned in PET-26 expression vector and finally, IPTG induced gene expression in E.coli BL-21 bacterium. It was shown that longer induction time led to more expression level. The recombinant enzyme has difference absorbance in 450 nm that confirmed the activity of this recombinant enzyme.
Authors and Affiliations
Mehdi Hassanshahian| Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran, Research Dept of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, IRAN, E-mail: hasanshahi@gmail.com, Mohammad Mehdi Yaghoobi| Research Dept of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, IRAN
Keywords
Related Articles
- EP ID EP12975
- DOI -
- Views 375
- Downloads 16
Prevalence of Concurrent Disorders of Ulnar Nerve Entrapment at the Elbow and Carpal Tunnel Syndrome Ahvaz Imam Khomeini Hospital During 2009 to 2012
Carpal tunnel syndrome (CTS) is the most well-known and frequent form of median nerve entrapment, and accounts for 90% of all entrapment neuropathies. Entrapment neuropathies of the ulnar nerve are relatively common wi...
Effect of gamma irradiation on morphological traits of three varieties of sesame crop in M1 generation (sesamum indicum L.)
The main objective for plant breeding is to increase genetic diversity. Mutation induction is a method to increase genetic diversity associated with selection, recombination, or a combination of these approaches in pla...
Effect of Sodium Chloride on Tropane Alkaloids Accumulation and Proline content in Datura metel and D. stramonium callus cultures
Plant tissue culture techniques were used to study the effect of NaCl on tropane alkaloids accumulation and proline content of both Datura metel and Datura stramonium. Callus cultures were established for both Datura s...
Investigation of Qualitative and Quantitative Effects of Drought and Salinity Stress on the Pennyroyal Plant
The effects of Drought and salinity stress on the growth and chemical compositions pennyroyal(Mentha pulegium L.) plant were investigated. This experiment was carried out in a factorial-randomized design with four replic...
CONTRACEPTIVE EFFICACY OF HYDRO-METHANOLIC FRUIT EXTRACT OF XYLOPIA AETHIOPICA IN MALE ALBINO RATS
The rising human population, particularly in the developing countries, and the attendant socioeconomic effects necessitates the need for its effective regulation. This study aims at exploring the contraceptive efficacy...